TTH DNA POLYMERASE

Product Name
TTH DNA POLYMERASE
Chemical Name
TTH DNA POLYMERASE
Synonyms
Q-THERMORT(TM);TTH DNA POLYMERASE;RTTH DNA POLYMERASE;THERMOSTABLE REVERSE TRANSCRIPTASE
CBNumber
CB7467709
Formula Weight
0
MOL File
Mol file
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TTH DNA POLYMERASE Property

storage temp. 
-20°C
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
11480022001
Product name
Tth DNA Polymerase
Packaging
500units
Price
$604
Updated
2024/03/01
American Custom Chemicals Corporation
Product number
ENZ0000868
Product name
TTH DNA POLYMERASE
Purity
95.00%
Packaging
5MG
Price
$495.36
Updated
2021/12/16
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TTH DNA POLYMERASE Chemical Properties,Usage,Production

Uses

Thermus thermophilus (Tth) DNA Polymerase might be used:

  • to amplify DNA fragments by polymerase chain reaction (PCR) due to its resistance towards prolonged incubations at high temperatures (95 °C)
  • to label DNA fragments with either radiolabeled nucleotides, digoxigenin, or biotin, since this enzyme accepts modified deoxyribonucleotides as substrates
  • to efficiently transcribe RNA targets into cDNA due to its intrinsic Mn-dependent reverse transcriptase (RT) activity
  • for real time PCR

General Description

Tth DNA Polymerase is isolated from the thermophilic eubacterium Thermus thermophilus HB8, and is purified to be free of nonspecific DNases and RNases. The enzyme is a highly processive 5′-3′ DNA polymerase, and lacks 3′-5′ exonuclease activity. The enzyme exhibits its highest activity at a pH of approximately 9 (adjusted at +25°C), and temperatures around +75°C. It is resistant to prolonged incubations at high temperatures (+95°C).

Biochem/physiol Actions

Thermus thermophilus (Tth) DNA Polymerase is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5′-3′ DNA polymerase and lacks 3′-5′ exonuclease activity (proof reading). It was found to possess a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions – much higher than that demonstrated for Escherichia coli DNA polymerase I and Taq DNA Polymerase. The RT activity is not associated with RNase H activity. The elevated temperatures of Tth DNA Polymerase (optimum +55°C to +70°C, maximum +95°C) activity overcomes the problems posed by RNA secondary structure. Resulting cDNA can be amplified by PCR using the same enzyme in the presence of Mg2+-ions. The ability of Tth DNA Polymerase to perform both reverse transcription and DNA amplification at elevated temperatures allows this enzyme to be used for quantitative RT-PCR, cloning, and gene expression analysis of cellular and viral RNA. Tth DNA Polymerase is used for RT-PCR amplification of RNA up to 1kb.

TTH DNA POLYMERASE Preparation Products And Raw materials

Raw materials

Preparation Products

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TTH DNA POLYMERASE Suppliers

Yinjia (Shanghai) Bio-pharmaceutical Technology Co. , Ltd.
Tel
021-52992719 13512169707
Email
product@yinjibio.com
Country
China
ProdList
29
Advantage
58
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51471
Advantage
80
Beijing Solarbio Science & Tecnology Co., Ltd.
Tel
010-50973130 4009686088
Email
3193328036@qq.com
Country
China
ProdList
29797
Advantage
68
Wuhan Peptide Core Biotechnology Co., Ltd
Tel
027-65317797 13667154760
Email
1096814016@qq.com
Country
China
ProdList
9916
Advantage
58