BCIP/NBT

Product Name: BCIP/NBT
Chemical Name: BCIP/NBT
Synonyms: NBT/BCIP;BCIP/NBT;BCIP/INT;BM PURPLE;BCIP/NBT PLUS;BCIP/NBT-BLUE;NBT-X-PHOSPHATE;SIGMAFAST? BCIP?/NBT;NBT-BCIP(R) solution;NBT/BCIP SUBSTRATE SOLN
CBNumber: CB5448175
Molecular Formula: C48H34BrClN11O10P
Formula Weight: 1071.18
MOL File: Mol file

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BCIP/NBT Property

Flash point:: 67 °C
storage temp.: -20°C
form: tablet
color: dark purple
Water Solubility: water: 10mL, clear, yellow

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 26
WGK Germany: 2

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: B1911
Product name: BCIP<SUP>®</SUP>/NBT Liquid Substrate System
Purity: ready to use solution
Packaging: 100 mL
Price: $111
Updated: 2025/07/31

Sigma-Aldrich

Product number: B1911
Product name: BCIP®/NBT Liquid Substrate System
Purity: ready to use solution
Packaging: 100mL
Price: $108
Updated: 2024/03/01

Sigma-Aldrich

Product number: B5655
Product name: SIGMAFAST™ BCIP®/NBT
Purity: tablet
Packaging: 5TABLETS
Price: $58.3
Updated: 2023/06/20

Sigma-Aldrich

Product number: B5655
Product name: SIGMAFAST™ BCIP®/NBT
Purity: tablet
Packaging: 25TABLETS
Price: $204
Updated: 2023/06/20

American Custom Chemicals Corporation

Product number: HCH0127002
Product name: 2-(4-IODOPHENYL)-5-(4-NITROPHENYL)-3-PHENYLTETRAZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT
Purity: 95.00%
Packaging: 5MG
Price: $499.85
Updated: 2021/12/16

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BCIP/NBT Chemical Properties,Usage,Production

Uses

SIGMAFAST BCIP^?/NBT has been used as a substrate

for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
for alkaline phosphatase in the histochemical staining of primary osteoblasts
for alkaline phosphatase staining in bone marrow stromal cells

Uses

A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.

General Description

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

BCIP/NBT Preparation Products And Raw materials

Raw materials

Preparation Products

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BCIP/NBT Suppliers

China 21 Germany 1 Japan 2 Switzerland 1 United Kingdom 1 United States 15 Global 41

Sigma-Aldrich

Tel: 021-61415566 800-8193336
Email: orderCN@merckgroup.com
Country: China
ProdList: 51395
Advantage: 80

Nantong Feiyu Biological Technology CO.,LTD

Tel: 0513-68819626; 15152877458
Fax: 0513-68669626
Email: feiyubio@163.com
Country: China
ProdList: 4817
Advantage: 58

Shanghai Yaji Biological Technology Co., Ltd.

Tel: 021-34661275 15301693058
Email: yajikit@163.com
Country: China
ProdList: 9897
Advantage: 58

Shanghai Aladdin Biochemical Technology Co.,Ltd.

Tel: 400-6206333 13167063860
Email: anhua.mao@aladdin-e.com
Country: China
ProdList: 48457
Advantage: 58

Merck Millipore

Tel: 1-400-889-1988 400-889-1988
Email: bioscience@merckgroup.com
Country: China
ProdList: 6839
Advantage: 58

Merck KGaA

Tel: 21-20338288
Email: ordercn@merckgroup.com
Country: China
ProdList: 6394
Advantage: 58

bioWORLD molecular life sciences

Tel: 1-614-792-8680
Email: customerservice@bio-world.com
Country: China
ProdList: 6128
Advantage: 58

Aladdin Scientific

Tel
Email: tp@aladdinsci.com
Country: United States
ProdList: 57505
Advantage: 58

Shenzhen Zuoke Biotechnology Co., Ltd.

Tel: 13532174601
Country: CHINA
ProdList: 109
Advantage: 58

Guangzhou Lucheng Biotechnology Co., Ltd.

Tel: 16620452357
Country: CHINA
ProdList: 0
Advantage: 58

BCIP/NBTRelated Search:

Uracil DNA Glycosylase (UDG) dNTP Mix 25mM each STREPTAVIDIN-HRP TTH DNA POLYMERASE Hotstart HiTaq Two-Step RT-PCR Mix Hotstart Master PCR Mix (25X) Hotstart HiTaq PCR Mix (2X) dN(U)TP Mix 20/40mM each Hotstart HiTaq DNA Polymerase Taq Antibody (10U/ul) Abstart Master PCR Mix (25X) Tth One-Step RT-PCR Mix Hotstart HiTaq One-Step RT-PCR Mix AP-Streptavidin Platinum Taq DNA Polymerase Abstart PCR Mix (2X) Hotstart HiTaq One-Step RT-PCR Mix (Heat-labile UDG)

BCIP/NBT

SIGMAFAST? BCIP?/NBT

NBT-BCIP(R) solution

BCIP(R)/NBT, NBT-X-phosphate

NBT-X-PHOSPHATE

NBT/BCIP WITH LEVAMISOL

NBT/BCIP TOLUIDINE SALT

NBT/BCIP SUBSTRATE SOLN

NBT/BCIP PLUS SUPPRESSOR

NBT/BCIP

NITRO BLUE TETRA-ZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT

NITRO-BLUE TETRAZOLIUM CHLORIDE, 5-BROMO-4-CHLORO-INDOLYLPHOSPHATE

BM PURPLE

BCIP/NBT STABILIZED CHROMOGEN

BCIP/NBT PLUS

BCIP/NBT LIQUID SUBSTRATE SYSTEM

BCIP/NBT-BLUE

BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE

BCIP/INT

INT/BCIP TOLUIDINE SALT

5-BROMO, 4-CHLORO, 3-INDOLYPHOSPHATE/NITROBLUETETRAZOLIUM

5-BROMO, 4-CHLORO, 3-INDOLYPHOSPHATE/IODONITROTETRAZOLIUM

5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE/P-IODONITROTETRAZOLIUM

5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE/NITROBLUE TETRAZOLIUM

5-BROMO-4-CHLORO-3-INDOLYLPHOSPHATE/IODONITROTETRAZOLIUM CHLORIDE

5-BROMO-4 CHLORO-3-INDOLYL-PHOSPHATE AND NITROBLUE TETRAZOLIUM

2-(4-IODOPHENYL)-5-(4-NITROPHENYL)-3-PHENYLTETRAZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT

1-STEP NBT/BCIP SOLUTION