BCIP/NBT

Product Name
BCIP/NBT
Chemical Name
BCIP/NBT
Synonyms
BCIP/NBT;BCIP/INT;NBT/BCIP;BM PURPLE;BCIP/NBT-BLUE;BCIP/NBT PLUS;NBT-X-PHOSPHATE;SIGMAFAST? BCIP?/NBT;NBT-BCIP(R) solution;INT/BCIP TOLUIDINE SALT
CBNumber
CB5448175
Molecular Formula
C48H34BrClN11O10P
Formula Weight
1071.18
MOL File
Mol file
More
Less

BCIP/NBT Property

Flash point:
67 °C
storage temp. 
-20°C
form 
tablet
More
Less

Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
26
WGK Germany 
2
More
Less

Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

More
Less

N-Bromosuccinimide Price

Sigma-Aldrich
Product number
B1911
Product name
BCIP®/NBT Liquid Substrate System
Purity
ready to use solution
Packaging
100mL
Price
$108
Updated
2024/03/01
Sigma-Aldrich
Product number
B5655
Product name
SIGMAFAST™ BCIP®/NBT
Purity
tablet
Packaging
5TABLETS
Price
$58.3
Updated
2023/06/20
Sigma-Aldrich
Product number
B5655
Product name
SIGMAFAST™ BCIP®/NBT
Purity
tablet
Packaging
25TABLETS
Price
$204
Updated
2023/06/20
American Custom Chemicals Corporation
Product number
HCH0127002
Product name
2-(4-IODOPHENYL)-5-(4-NITROPHENYL)-3-PHENYLTETRAZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT
Purity
95.00%
Packaging
5MG
Price
$499.85
Updated
2021/12/16
More
Less

BCIP/NBT Chemical Properties,Usage,Production

Uses

SIGMAFAST BCIP?/NBT has been used as a substrate

  • for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting
  • for alkaline phosphatase in the histochemical staining of primary osteoblasts
  • for alkaline phosphatase staining in bone marrow stromal cells

Uses

A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.

General Description

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.

Appearance prior to use: light yellow color;
After color development: dark purple

BCIP/NBT Preparation Products And Raw materials

Raw materials

Preparation Products

More
Less

BCIP/NBT Suppliers

China 6 Germany 1 Japan 2 Switzerland 1 United Kingdom 1 United States 15 Global 26
Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
Country
China
ProdList
51471
Advantage
80
Nantong Feiyu Biological Technology CO.,LTD
Tel
0513-68819626; 13813788405
Fax
0513-68669626
Email
feiyubio2@163.com
Country
China
ProdList
4821
Advantage
58
Shanghai Yaji Biological Technology Co., Ltd.
Tel
021-34661275 15301693058
Email
yajikit@163.com
Country
China
ProdList
6633
Advantage
58
Shanghai Aladdin Biochemical Technology Co.,Ltd.
Tel
+86-18521732826
Email
market@aladdin-e.com
Country
China
ProdList
48617
Advantage
58
Aladdin Scientific
Tel
+1-833-552-7181
Email
sales@aladdinsci.com
Country
United States
ProdList
57511
Advantage
58

BCIP/NBTRelated Search:

Uracil DNA Glycosylase (UDG) dNTP Mix 25mM each STREPTAVIDIN-HRP TTH DNA POLYMERASE Hotstart HiTaq Two-Step RT-PCR Mix Hotstart Master PCR Mix (25X) Hotstart HiTaq PCR Mix (2X) dN(U)TP Mix 20/40mM each Hotstart HiTaq DNA Polymerase Taq Antibody (10U/ul) Abstart Master PCR Mix (25X) Tth One-Step RT-PCR Mix Hotstart HiTaq One-Step RT-PCR Mix AP-Streptavidin Platinum Taq DNA Polymerase Abstart PCR Mix (2X) Hotstart HiTaq One-Step RT-PCR Mix (Heat-labile UDG)

  • 1-STEP NBT/BCIP SOLUTION
  • 2-(4-IODOPHENYL)-5-(4-NITROPHENYL)-3-PHENYLTETRAZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT
  • 5-BROMO-4 CHLORO-3-INDOLYL-PHOSPHATE AND NITROBLUE TETRAZOLIUM
  • 5-BROMO-4-CHLORO-3-INDOLYLPHOSPHATE/IODONITROTETRAZOLIUM CHLORIDE
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE/NITROBLUE TETRAZOLIUM
  • 5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE/P-IODONITROTETRAZOLIUM
  • 5-BROMO, 4-CHLORO, 3-INDOLYPHOSPHATE/IODONITROTETRAZOLIUM
  • 5-BROMO, 4-CHLORO, 3-INDOLYPHOSPHATE/NITROBLUETETRAZOLIUM
  • INT/BCIP TOLUIDINE SALT
  • BCIP/INT
  • BCIP/NBT
  • BCIP/NBT ALKALINE PHOSPHATASE SUBSTRATE
  • BCIP/NBT-BLUE
  • BCIP/NBT LIQUID SUBSTRATE SYSTEM
  • BCIP/NBT PLUS
  • BCIP/NBT STABILIZED CHROMOGEN
  • BM PURPLE
  • NITRO-BLUE TETRAZOLIUM CHLORIDE, 5-BROMO-4-CHLORO-INDOLYLPHOSPHATE
  • NITRO BLUE TETRA-ZOLIUM CHLORIDE AND 5-BROMO-4-CHLORO-3-INDOLYL-PHOSPHATE, TOLUIDINE-SALT
  • NBT/BCIP
  • NBT/BCIP PLUS SUPPRESSOR
  • NBT/BCIP SUBSTRATE SOLN
  • NBT/BCIP TOLUIDINE SALT
  • NBT/BCIP WITH LEVAMISOL
  • NBT-X-PHOSPHATE
  • BCIP(R)/NBT, NBT-X-phosphate
  • NBT-BCIP(R) solution
  • SIGMAFAST? BCIP?/NBT