PO-PRO 3

PO-PRO 3 Property

Melting point:

>250℃

solubility 

Soluble in dimethyl sulfoxide

form 

powder

color 

Yellow-brown

Appearance

red solution

λmax

539 nm

ex/em

539/567 nm

ε(extinction coefficient)

87900 cm−1M−1

Φ(quantum yield)

0.57

Biological Applications

Nucleic acid hybridization; detecting nucleic acids,cells; nucleic acid fingerprinting; nucleic acid fragment sizing; nucleic acid amplification

Hazard and Precautionary Statements (GHS)

PO-PRO 3 Chemical Properties,Usage,Production

Description

PO-TAP-3 (Oxazole Orange Monomer, also known as PO-PRO^®-3) is a yellow fluorescent carbocyanine monomeric dye. PO-TAP-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.

The bright fluorescence signal and low background make PO-TAP-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

The dye is used to image cell nuclei and cytoplasm, label bacteria and sperms, count reticulocytes, perform nucleic acid amplification and hybridization, and as a temperature sensor.

Chemical Properties

ex/em: 539/567 nm
Extinction coefficient (ε): 87900 cm??1M??1
Quantum yield (Φ): 0.57

Definition

ChEBI: Po-Pro-3 is a cyanine dye and an organic iodide salt. It has a role as a fluorochrome. It contains a Po-Pro-3(2+).

Abs/Em Maxima

531 (complex)/568 (complex) nm