2-[(2-amino-5-methylph2-[(2-amino-5-methylphenoxy)methyl]-6-methoxy-8-aminoquinoline-n,n,n',n'-tetraacetic acid tetrapotassium saltenoxy)methyl]-6-methoxy-8-aminoquinoline-n,n,n Property

Melting point:

>250℃

RTECS 

MB8303000

storage temp. 

2-8°C

solubility 

H2O: soluble10mg/mL

form 

powder

color 

Light yellow

Water Solubility 

Soluble in water at 10mg/ml

λmax

353 nm, 333 nm

Merck 

13,8130

BRN 

5374262

Biological Applications

Calcium indicator; cadmium indicator; iron indicator; lanthanum indicator; zinc indicator; identifying taste modulators

Safety

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

F 

3-8-10

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P304+P340IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P405Store locked up.

2-[(2-amino-5-methylph2-[(2-amino-5-methylphenoxy)methyl]-6-methoxy-8-aminoquinoline-n,n,n',n'-tetraacetic acid tetrapotassium saltenoxy)methyl]-6-methoxy-8-aminoquinoline-n,n,n Chemical Properties,Usage,Production

Description

Quin-2 is a high-affinity fluorescent calcium indicator (K_d = 115 nM for calcium). It displays high selectivity for calcium, as it is not affected by sodium gradients, membrane potential, or intracellular pH. High affinity probes like quin-2 are ideal for monitoring low levels of calcium, as are found in resting cells. Loadings of up to 2 mM quin-2 are without serious toxic effects, so quin-2 may be used to buffer intracellular calcium transients. Excitation/emission maxima for quin-2 are 339 and 492 nm, respectively.

Uses

2-[(2-amino-5-methylphenoxy)methyl]-6-methoxy-8-aminoquinoline-n,n,n',n'-tetraacetic acid tetrapotassium salt can be used as a fluorescent highly selective Ca2+ indicator exhibiting fast uptake and release of Ca2+. Used as a calibration substance in the measurement of cytoplasmic free calcium.

Uses

QUIN 2, Tetrapotassium Salt is a useful fluorescing calcium indicator. It can also be used in biological study of practical aspects of measuring intracellular calcium signals with fluorescent indicators.

General Description

Binding of Ca²⁺ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).

Biochem/physiol Actions

Primary TargetCalcium Probes