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CATALASE ASSAY KIT

Product Name
CATALASE ASSAY KIT
Chemical Name
CATALASE ASSAY KIT
Synonyms
CATALASE ASSAY KIT
CBNumber
CB6509833
Formula Weight
0
MOL File
Mol file
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CATALASE ASSAY KIT Property

storage temp. 
2-8°C
Specific Activity
0.25 - 4nmol/min⋅mL
Major Application
pharmaceutical
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Safety

WGK Germany 
WGK 2
Storage Class
10 - Combustible liquids
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
219265
Product name
Catalase Assay Kit
Packaging
1kit
Price
$844
Updated
2025/07/31
Cayman Chemical
Product number
707002
Product name
Catalase Assay Kit
Packaging
96Well
Price
$315
Updated
2024/03/01
Cayman Chemical
Product number
707002
Product name
Catalase Assay Kit
Packaging
480Well
Price
$569
Updated
2024/03/01
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CATALASE ASSAY KIT Chemical Properties,Usage,Production

Description

Catalase (EC 1.11.1.6; 2H2O2 oxidoreductase) is an ubiquitous antioxidant enzyme that is present in most aerobic cells. Catalase (CAT) is involved in the detoxification of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), which is a toxic product of both normal aerobic metabolism and pathogenic ROS production. This enzyme catalyzes the conversion of two molecules of H2O2 to molecular oxygen and two molecules of water (catalytic activity). CAT also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. While the aliphatic alcohols serve as specific substrates for CAT, other enzymes with peroxidatic activity do not utilize these substrates. In humans, the highest levels of catalase are found in liver, kidney, and erythrocytes, where it is believed to account for the majority of hydrogen peroxide decomposition. The Cayman Chemical Catalase Assay Kit utilizes the peroxidatic function of CAT for determination of enzyme activity. The method is based on the reaction of the enzyme with methanol in the presence of an optimal concentration of H2O2. The formaldehyde produced is measured spectrophotometrically with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald) as the chromogen. Purpald specifically forms a bicyclic heterocycle with aldehydes, which upon oxidation changes from colorless to a purple color. The assay can be used to measure CAT activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates.

References

[1] LARS H. JOHANSSON  L. A H B. A spectrophotometric method for determination of catalase activity in small tissue samples[J]. Analytical biochemistry, 1988, 174 1: Pages 331-336. DOI: 10.1016/0003-2697(88)90554-4
[2] CONRAD R. WHEELER. Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity[J]. Analytical biochemistry, 1990, 184 2: Pages 193-199. DOI: 10.1016/0003-2697(90)90668-y
[3] CONG CONG. Sinigrin attenuates angiotensin II?induced kidney injury by inactivating nuclear factor?κB and extracellular signal?regulated kinase signaling in vivo and in vitro.[J]. International journal of molecular medicine, 2021, 48 2. DOI: 10.3892/ijmm.2021.4994
[4] HAMID ARAZI  Abbas A  Heidar Mohammadjafari. Use of anabolic androgenic steroids produces greater oxidative stress responses to resistance exercise in strength-trained men[J]. Toxicology Reports, 2017, 4: Pages 282-286. DOI: 10.1016/j.toxrep.2017.05.005
[5] KAMAL DATTA. Exposure to heavy ion radiation induces persistent oxidative stress in mouse intestine.[J]. PLoS ONE, 2012: e42224. DOI: 10.1371/journal.pone.0042224

CATALASE ASSAY KIT Preparation Products And Raw materials

Raw materials

Preparation Products

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CATALASE ASSAY KIT Suppliers

amyjet
Tel
4006800868;027-59626688 18371453821;18771149750
Email
sales@amyjet.com
Country
China
ProdList
2917
Advantage
58
Tanmo Quality Inspection Technology Co., Ltd.
Tel
4008-099-669
Email
23419001name@qq.com
Country
CHINA
ProdList
6043
Advantage
58
Merck Millipore
Tel
1-400-889-1988 400-889-1988
Email
bioscience@merckgroup.com
Country
China
ProdList
6839
Advantage
58
Cayman Chemical Company
Tel
800-364-9897
Email
sales@caymanchem.com
Country
China
ProdList
6838
Advantage
58
Merck KGaA
Tel
21-20338288
Email
ordercn@merckgroup.com
Country
China
ProdList
6394
Advantage
58
Zhejiang Haonuo Biotechnology Co., Ltd.
Tel
13910790812
Country
CHINA
ProdList
169
Advantage
58
Guangzhou Biopark Technology Co., Ltd.
Tel
020-83808789
Country
CHINA
ProdList
179
Advantage
58
Langfang Yusheng Ruichuang Technology Co., Ltd.
Tel
17398520978
Country
CHINA
ProdList
157
Advantage
58
Guangzhou Foresight Technology Co., Ltd.
Tel
13113368386
Country
CHINA
ProdList
113
Advantage
58
Aoki Biotechnology (Shenzhen) Co., Ltd.
Tel
18566777408
Country
CHINA
ProdList
156
Advantage
58

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