MITOCHONDRIA STAINING KIT Property

storage temp. 

2-8°C

Safety

Hazard Codes 

Xn

Risk Statements 

21/22

Safety Statements 

36-36/37

WGK Germany 

3

Hazard and Precautionary Statements (GHS)

MITOCHONDRIA STAINING KIT Chemical Properties,Usage,Production

Uses

The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.

General Description

In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.