chlorothricin Property

Melting point:

206-207°

Boiling point:

1050.8±65.0 °C(Predicted)

Density 

1.39±0.1 g/cm3(Predicted)

storage temp. 

Store at -20°C

solubility 

Soluble in ethanol;Soluble in methanol;Soluble in DMSO;Soluble in dimethyl formamide

form 

solid

pka

pK: 5.01, 7.91(at 25℃)

color 

white

Hazard and Precautionary Statements (GHS)

chlorothricin Chemical Properties,Usage,Production

Uses

Chlorothricin is a spirotetronic acid antibiotic inhibitor of pyruvate carboxylase.

Uses

The tetronic acid, chlorothricin, is an unusual macrocyclic antibiotic from a Streptomyces sp., related to kijanimicin, saccharocarcins, tetrocarcins and versipelostatin. Chlorothricin inhibits cholesterol biosynthesis from mevalonate, and inhibits pyruvate carboxylases purified from rat liver, chicken liver and Azotobacter vinelandii.

Biological Activity

chlorothricin is a macrolide-type antibiotic.macrolides, a class of natural products belonging to the polyketide class of natural products, consist of a large macrocyclic lactone ring. the lactone rings are oftem 14-, 15-, or 16-membered. some macrolides have been reported to have antibiotic or antifungal activity and are widely used as pharmaceutical drugs.

in vitro

in a previous study, chlorothricin was found to inhibit the reaction catalyzed by pyruvate carboxylase from bacillus stearothermophilus. moreover, with steady-state kinetic measurements, inhibition of the overall reaction was found to be competitive with the allosteric activator of this enzyme, coasac, and non-competitive with respect to both substrates of mgatp and pyruvate. these findings strongly indicated that the site 1conformation of pyruvate carboxylase responsible for the regulation of the overall enzyme activity could be influenced by chlorothricin and coasac in an antagonistic manner [1].

IC 50

173, 500, 260, and 120 μm for pyruvate carboxylases from bacillus, azotobacter, rat, and chicken, respectively.

References

[1] schindler pw, zhner h. mode of action of the macrolide-type antibiotic, chlorothricin. kinetic study of the inhibition of pyruvate carboxylase from bacillus stearothermophilus. eur j biochem. 1973 nov 15;39(2):591-600.