PHTHALAZIN-5-AMINE

PHTHALAZIN-5-AMINE Property

Melting point:

223-224 °C(Solv: methanol (67-56-1))

Boiling point:

423.2±20.0 °C(Predicted)

Density 

1.292±0.06 g/cm3(Predicted)

storage temp. 

Keep in dark place,Inert atmosphere,Room temperature

pka

5.32±0.30(Predicted)

Appearance

Light yellow to brown Solid

Safety

HS Code 

2933998090

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

PHTHALAZIN-5-AMINE Chemical Properties,Usage,Production

Uses

5-Aminophthalazine is a building block that was used as a reactant in the synthetic preparation of ezrin inhibitors targeting metastatic osteosarcoma.

Synthesis

General procedure for the synthesis of 5-amino phthalazine using 5-nitro phthalazine as starting material: 0.88 g (5.0 mmol) of 5-nitro phthalazine was dissolved in 30 mL of tetrahydrofuran at 50 °C, followed by the addition of 4.37 g (25.1 mmol) of sodium bisulfite dissolved in a solution of 15 mL of water. The reaction mixture was stirred continuously at 50 °C for 15 min. Upon completion of the reaction, the organic phase was extracted with ethyl acetate (3 x 100 mL) and the combined organic phases were washed with brine (50 mL) and dried with anhydrous magnesium sulfate. After filtration, the solvent was removed by concentration under reduced pressure to give the crude product as a bright yellow precipitate. The crude product was recrystallized in hot methanol to give 0.49 g (67% yield) of purified 5-aminophthalazine. The structure of the product was confirmed by 1H NMR (400 MHz, DMSO-d6) and 13C NMR (100.17 MHz, DMSO-d6) as follows: 1H NMR δ: 9.80 (s, 1H), 9.60 (s, 1H), 7.60 (dd, J = 4.6, 7.6 Hz, 1H), 7.20 (dd, J = 1.9, 4.6 Hz, 1H), 7.02 (d, J = 7.6 Hz, 1H), 6.50 (s, 2H); 13C NMR δ: 150.1,146.5,145.1,133.2,126.1,114.7,114.1,112.2.

References

[1] Journal of Medicinal Chemistry, 1986, vol. 29, # 8, p. 1329 - 1340
[2] Bioorganic and Medicinal Chemistry, 2014, vol. 22, # 1, p. 478 - 487
[3] Journal of Medicinal Chemistry, 2005, vol. 48, # 3, p. 744 - 752
[4] Bioorganic and Medicinal Chemistry Letters, 2007, vol. 17, # 9, p. 2577 - 2580
[5] Patent: US2006/116368, 2006, A1. Location in patent: Page/Page column 35; 36