3X FLAG PEPTIDE

ChemicalBook > CAS DataBase List > 3X FLAG PEPTIDE

Product Name: 3X FLAG PEPTIDE
CAS No.: 402750-12-3
Chemical Name: 3X FLAG PEPTIDE
Synonyms: 3×FLAG peptide;3X FLAG PEPTIDE;3X FLAG(R) Peptide;MDYKDHDGDYKDHDIDYKDDDDK;3X Flag Peptide Trifluoroacetate;Flag Peptide trifluoroacetate salt;MET-ASP-TYR-LYS-ASP-HIS-ASP-GLY-ASP-TYR-LYS-ASP-HIS-ASP-ILE-ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS;3X FLAG peptide,Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys,MDYKDHDGDYKDHDIDYKDDDDK;L-Lysine, L-methionyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartylglycyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartyl-L-isoleucyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-
CBNumber: CB7463716
Molecular Formula: C120H169N31O49S
Formula Weight: 2861.9
MOL File: 402750-12-3.mol

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3X FLAG PEPTIDE Property

Boiling point:: 2863.6±65.0 °C(Predicted)
Density: 1.458±0.06 g/cm3(Predicted)
storage temp.: 2-8°C
solubility: ≥25mg/ml in TBS (0.5M Tris-HCl, pH 7.4, with 1M NaCl), 1X TBS as recommended buffer.
form: lyophilized powder
color: White to off-white

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Safety

WGK Germany: 3

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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: F4799
Product name: 3X FLAG® Peptide
Purity: lyophilized powder
Packaging: 4MG
Price: $459
Updated: 2024/03/01

Sigma-Aldrich

Product number: F4799
Product name: 3X FLAG® Peptide
Purity: lyophilized powder
Packaging: 25MG
Price: $2130
Updated: 2024/03/01

ApexBio Technology

Product number: A6001
Product name: 3X FLAG Peptide
Packaging: 4mg
Price: $90
Updated: 2021/12/16

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3X FLAG PEPTIDE Chemical Properties,Usage,Production

Biological Activity

the dykddddk-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1. the dykddddk peptide binds to the antibody m1. whether binding is calcium-dependent manner2 or –independent3 remains controversial. a disadvantage of the system is that the monoclonalantibody purification matrix is not as stable as others. in general, small tags can be detected with specific monoclonal antibodies.to improve the detection of the dykddddk tag the 3x (dykddddk) system has been developed. this threetandem dykddddk epitope is hydrophilic, 22-amino-acids long and can detect up to 10 fmol of expressed fusion protein. the dykddddk-tagged maltodextrin-binding protein of pyrococcus furiosus has been crystallized4 and the quality of the crystals was very similar to that of crystals of untagged protein.finally, the dykddddk-tag can be removed by treatment with enterokinase, which is specific for the five c-terminal amino acids of the peptide sequence5.

References

1. hopp tp, prickett ks, price vl, libby rt, march cj, ceretti dp, urdal dl, conlon pj (1988) a short polypeptide marker sequence useful for recombinant protein identification and purification. bio/technology 6:1204–1210.2. hopp tp, gallis b, prikett ks (1996) metal-binding properties of a calcium dependent monoclonal antibody. mol immunol 33:601–608.3. einhauer a, jungbauer a (2000) kinetics and thermodynamical properties of the monoclonal antibody m1 directed against the flag peptide. 20th international symposium on the separation of proteins, peptides, and polynucleotides (isppp). lublijana, slovenia, november 5–8, 2000.4. bucher mh, evdokimov ag, waugh ds (2002) differential effects of short affinity tags on the crystallization of pyrococcus furiosus maltodextrin-binding protein. biol cryst 58:392–397.5. maroux s, baratti j, desnuelle p (1971) purification and specificity of procine enterokinase. j biol chem 246:5031–5039.

3X FLAG PEPTIDE Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from 3X FLAG PEPTIDE manufacturers

Dorne Chemical Technology co. LTD

Product: 3X FLAG PEPTIDE 402750-12-3
Price: US $1.00/g
Min. Order: 1g
Purity: 99%
Supply Ability: 100kg
Release date: 2024-03-26

Career Henan Chemica Co

Product: 3X FLAG PEPTIDE 402750-12-3
Price: US $1.00/KG
Min. Order: 1KG
Purity: 85.0-99.8%
Supply Ability: 20 tons
Release date: 2020-11-09

Hong Kong Excellence Biotechnology Co., Ltd.

Product: 3 x flag
Price: US $50.00/box
Min. Order: 1box
Purity: >99%
Supply Ability: 20tons
Release date: 2023-10-12

402750-12-3, 3X FLAG PEPTIDERelated Search:

DNA, SINGLE STRANDED, IMM. ON CELLULOSE, FROM CALF THYMUS*

3X FLAG(R) Peptide

3X FLAG peptide,Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys,MDYKDHDGDYKDHDIDYKDDDDK

MET-ASP-TYR-LYS-ASP-HIS-ASP-GLY-ASP-TYR-LYS-ASP-HIS-ASP-ILE-ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS

3X FLAG PEPTIDE

MDYKDHDGDYKDHDIDYKDDDDK

L-Lysine, L-methionyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartylglycyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartyl-L-isoleucyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-

Flag Peptide trifluoroacetate salt

3×FLAG peptide

3X Flag Peptide Trifluoroacetate

402750-12-3

C120H169N31O49S

BioChemical

Protein Purification

Plant Biotechnology

Plant Proteomics

FLAG Affinity Purification

EpitopeTags