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3X FLAG PEPTIDE

Product Name
3X FLAG PEPTIDE
CAS No.
402750-12-3
Chemical Name
3X FLAG PEPTIDE
Synonyms
3×FLAG peptide;3X FLAG PEPTIDE;3X FLAG(R) Peptide;MDYKDHDGDYKDHDIDYKDDDDK;3X Flag Peptide Trifluoroacetate;Flag Peptide trifluoroacetate salt;MET-ASP-TYR-LYS-ASP-HIS-ASP-GLY-ASP-TYR-LYS-ASP-HIS-ASP-ILE-ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS;3X FLAG peptide,Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys,MDYKDHDGDYKDHDIDYKDDDDK;L-Lysine, L-methionyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartylglycyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartyl-L-isoleucyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-
CBNumber
CB7463716
Molecular Formula
C120H169N31O49S
Formula Weight
2861.9
MOL File
402750-12-3.mol
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3X FLAG PEPTIDE Property

Boiling point:
2863.6±65.0 °C(Predicted)
Density 
1.458±0.06 g/cm3(Predicted)
storage temp. 
2-8°C
solubility 
≥25mg/ml in TBS (0.5M Tris-HCl, pH 7.4, with 1M NaCl), 1X TBS as recommended buffer.
form 
lyophilized powder
color 
White to off-white
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Safety

WGK Germany 
3
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Hazard and Precautionary Statements (GHS)

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
F4799
Product name
3X FLAG® Peptide
Purity
lyophilized powder
Packaging
4MG
Price
$459
Updated
2024/03/01
Sigma-Aldrich
Product number
F4799
Product name
3X FLAG® Peptide
Purity
lyophilized powder
Packaging
25MG
Price
$2130
Updated
2024/03/01
ApexBio Technology
Product number
A6001
Product name
3X FLAG Peptide
Packaging
4mg
Price
$90
Updated
2021/12/16
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3X FLAG PEPTIDE Chemical Properties,Usage,Production

Biological Activity

the dykddddk-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1. the dykddddk peptide binds to the antibody m1. whether binding is calcium-dependent manner2 or –independent3 remains controversial. a disadvantage of the system is that the monoclonalantibody purification matrix is not as stable as others. in general, small tags can be detected with specific monoclonal antibodies.to improve the detection of the dykddddk tag the 3x (dykddddk) system has been developed. this threetandem dykddddk epitope is hydrophilic, 22-amino-acids long and can detect up to 10 fmol of expressed fusion protein. the dykddddk-tagged maltodextrin-binding protein of pyrococcus furiosus has been crystallized4 and the quality of the crystals was very similar to that of crystals of untagged protein.finally, the dykddddk-tag can be removed by treatment with enterokinase, which is specific for the five c-terminal amino acids of the peptide sequence5.

References

1. hopp tp, prickett ks, price vl, libby rt, march cj, ceretti dp, urdal dl, conlon pj (1988) a short polypeptide marker sequence useful for recombinant protein identification and purification. bio/technology 6:1204–1210.2. hopp tp, gallis b, prikett ks (1996) metal-binding properties of a calcium dependent monoclonal antibody. mol immunol 33:601–608.3. einhauer a, jungbauer a (2000) kinetics and thermodynamical properties of the monoclonal antibody m1 directed against the flag peptide. 20th international symposium on the separation of proteins, peptides, and polynucleotides (isppp). lublijana, slovenia, november 5–8, 2000.4. bucher mh, evdokimov ag, waugh ds (2002) differential effects of short affinity tags on the crystallization of pyrococcus furiosus maltodextrin-binding protein. biol cryst 58:392–397.5. maroux s, baratti j, desnuelle p (1971) purification and specificity of procine enterokinase. j biol chem 246:5031–5039.

3X FLAG PEPTIDE Preparation Products And Raw materials

Raw materials

Preparation Products

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3X FLAG PEPTIDE Suppliers

SIGMA-RBI
Tel
--
Fax
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Country
Switzerland
ProdList
6896
Advantage
91
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View Lastest Price from 3X FLAG PEPTIDE manufacturers

Dorne Chemical Technology co. LTD
Product
3X FLAG PEPTIDE 402750-12-3
Price
US $1.00/g
Min. Order
1g
Purity
99%
Supply Ability
100kg
Release date
2024-03-26
Career Henan Chemica Co
Product
3X FLAG PEPTIDE 402750-12-3
Price
US $1.00/KG
Min. Order
1KG
Purity
85.0-99.8%
Supply Ability
20 tons
Release date
2020-11-09
Hong Kong Excellence Biotechnology Co., Ltd.
Product
3 x flag
Price
US $50.00/box
Min. Order
1box
Purity
>99%
Supply Ability
20tons
Release date
2023-10-12

402750-12-3, 3X FLAG PEPTIDERelated Search:


  • 3X FLAG(R) Peptide
  • 3X FLAG peptide,Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys,MDYKDHDGDYKDHDIDYKDDDDK
  • MET-ASP-TYR-LYS-ASP-HIS-ASP-GLY-ASP-TYR-LYS-ASP-HIS-ASP-ILE-ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
  • 3X FLAG PEPTIDE
  • MDYKDHDGDYKDHDIDYKDDDDK
  • L-Lysine, L-methionyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartylglycyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-histidyl-L-α-aspartyl-L-isoleucyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-
  • Flag Peptide trifluoroacetate salt
  • 3×FLAG peptide
  • 3X Flag Peptide Trifluoroacetate
  • 402750-12-3
  • C120H169N31O49S
  • BioChemical
  • Protein Purification
  • Plant Biotechnology
  • Plant Proteomics
  • FLAG Affinity Purification
  • EpitopeTags