CI 51180 Property

Melting point:

>300 °C (dec.)(lit.)

storage temp. 

room temp

solubility 

Soluble in water, ethanol

Colour Index 

51180

form 

Powder

color 

Dark green to black

PH Range

10.1(BLUE)-11.1(RED)

Water Solubility 

Soluble in ethanol, and water (50 g/L) at 25°C.

λmax

633 nm

Biological Applications

Detecting microorganisms; treating virus infectious diseases; photodynamic therapy

Safety

Hazard Codes 

Xi

Safety Statements 

22-24/25

WGK Germany 

3

RTECS 

DJ1931000

HS Code 

3212900000

Toxicity

mouse,LD,intraperitoneal,> 500mg/kg (500mg/kg),"Summary Tables of Biological Tests," National Research Council Chemical-Biological Coordination Center. Vol. 4, Pg. 46, 1952.

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust/fume/gas/mist/vapours/spray.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

CI 51180 Chemical Properties,Usage,Production

Chemical Properties

dark green to black powder

Uses

Nile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites.

Uses

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Uses

Nile Blue A is a stain used for discriminating between melanins and lipofuscins in paraffin sections of animal tissue

General Description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Purification Methods

Crystallise the dye from aqueous AcOH. It has UV: at 630nm (96% aqueous max EtOH) and 635nm (H2O). The betaine has UV: at 513nm (EtOH). [Crossley et al. J Am Chem Soc 74 max 57, 578 1952, Merill & Spencer J Am Chem Soc 70 3683 1948, Beilstein 27 II 457, 27 III/IV 5166.]