IMMOBILIZED PROTEIN A/G
Description Source Background- Product Name
- IMMOBILIZED PROTEIN A/G
- Chemical Name
- IMMOBILIZED PROTEIN A/G
- Synonyms
- PROTEIN A/G;PROTEIN A/G-AGAROSE;IMMOBILIZED PROTEIN A/G;RECOMBINANT PROTEIN A/G;IMMOBILIZED PROTEIN A/G PLUS;ULTRALINK(TM) IMMOBILIZED PROTEIN G;ULTRALINK(TM) IMMOBILIZED PROTEIN A/G;ULTRALINK(TM) IMMOBILIZED PROTEIN A/G PLUS
- CBNumber
- CB8133454
- Formula Weight
- 0
- MOL File
- Mol file
IMMOBILIZED PROTEIN A/G Property
- storage temp.
- 0-6°C
IMMOBILIZED PROTEIN A/G Chemical Properties,Usage,Production
Description
The recombinant Protein A/G consists of 5 IgG-binding regions of protein A and 2 of protein G, which corresponds to the Protein A and G domains that are included in the recombinant sequence. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G to ensure the maximum specific IgG binding. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C1 and C3. The fusion protein has a predicted molecular mass of 47.7kDa and containing 429 amino acids.
Source
Escherichia coli
Background
Recombinant Protein A/G fusion protein joins IgG binding domains of both Protein A and Protein G.
Protein A/G includes four Fc binding domains from Protein A and two from Protein G, yielding a final mass of 50.4 kDa. The binding dependency to pH of Protein A/G lower than Protein A, but has the additive properties of Protein A and G together. Protein A/G binds to all subclasses of human IgG, making it helpful for purifying polyclonal or monoclonal IgG antibodies whose subclasses have not been identifieed. Protein A/G binds to IgA, IgE, IgM and IgD. Protein A/G binds to all subclasses of mouse IgG excluding mouse IgA, IgM or serum albumin. This permits Protein A/G to be used in purification and detection of mouse monoclonal IgG antibodies, with no interference from IgA, IgM and serum albumin. Mouse monoclonal antibodies normally have a stronger affinity to the chimeric Protein A/G than to either Protein A or Protein G. Protein A/G also has been used for purification of macaque IgG.
IMMOBILIZED PROTEIN A/G Preparation Products And Raw materials
Raw materials
Preparation Products
IMMOBILIZED PROTEIN A/G Suppliers
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