ChemicalBook > CAS DataBase List > 6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]

6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]

Product Name: 6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]
CAS No.: 877049-90-6
Chemical Name: 6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]
Synonyms: 6-TET CE Phosphoramidite;TET phosphoramidite, 6-isomer;5'-Tetrachlorofluorescein CEP;5'-Tetrachlorofluorescein phosphoramidite;TET Dye Amidite (5'-Tetrachloro-Fluorescein Phosphoramidite);6-tet phosphoramidite [5'-tetrachlorofluorescein phosphoramidite;6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite];6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*;6-(4,7,2', 7'-Tetrachloro-3', 6'-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyI)-(N, N-diisopropyD)]-phosphoramidite;6-(4, 7, 2', 7'-Tetrachloro-3', 6'-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyl)-(N, N-diisopropyl)]-phosphoramidite)
CBNumber: CB83382420
Molecular Formula: C46H54Cl4N3O10P
Formula Weight: 981.72
MOL File: 877049-90-6.mol

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6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] Property

Boiling point:: 895.1±65.0 °C(Predicted)
solubility: Good solubility in acetonitrile and DCM
pka: 12.78±0.20(Predicted)
Appearance: white solid foam

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Hazard and Precautionary Statements (GHS)

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6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] Chemical Properties,Usage,Production

Description

TET phosphoramidite for synthesis of fluorescently labeled oligonucleotides, pure 6-isomer.

TET (tetrachlorofluorescein) is a green-fluorescent fluorescein derivate (absorption maximum at 519 nm, emission maximum at 535 nm).

TET phosphoramidite is used for synthesis of fluorescently-labeled primers and hybridization probes for qPCR. TET can be used with DusQ1 fluorescence quencher (can be used with 500 Å DusQ1 CPG 500).

5’-labeled primers are used with non-labeled reverse primers for microsatellite amplification via PCR followed by fragment analysis. TET-labeled amplification products can be analyzed using various sequencers for capillary electrophoresis, including ABI PRISM^® 310 Genetic Analyzer.

Condensation: 3 min.

Deprotection: standard conditions with 25% ammonium hydroxide; deprotection time depends on oligonucleotide composition and nucleobase protecting groups (deprotection for 17 hours at 55°C removes all protecting groups from standard nucleobases). AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) can be used with ~5% non-fluorescent side product forming. To avoid formation of the side product, start deprotection with ammonium hydroxide (30 min at room temperature), then add an equal volume of 40% aqueous methylamine and continue deprotection as required with AMA (10 min at 65°C).

Uses

6-TET phosphoramidite (5′-Tetrachlorofluorescein phosphoramidite) is a fluorescent dye that can be used for labeling an oligonucleotide with fluorescein[1].

References

[1] El-Hajj HH, et al. Detection of rifampin resistance in Mycobacterium tuberculosis in a single tube with molecular beacons. J Clin Microbiol. 2001 Nov;39(11):4131-7. DOI:10.1128/JCM.39.11.4131-4137.2001

Abs/Em Maxima

519/535 nm

Fluoresene quantum yield

0.47

Extinction Coefficient

100000

CF260

0.17

CF280

0.09

6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] Preparation Products And Raw materials

Raw materials

Preparation Products

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877049-90-6, 6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]Related Search:

5'(6)-FAM Propanoic acid, 2,2-dimethyl-, 1,1'-[6-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-2',4,4',5',7,7'-hexachloro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl] ester

6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite]

6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*

5'-Tetrachlorofluorescein CEP

6-(4,7,2', 7'-Tetrachloro-3', 6'-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyI)-(N, N-diisopropyD)]-phosphoramidite

TET phosphoramidite, 6-isomer

Propanoic acid, 2,2-dimethyl-, 6-[10-[bis(1-methylethyl)amino]-13-cyano-1-oxo-9,11-dioxa-2-aza-10-phosphatridec-1-yl]-2',4,7,7'-tetrachloro-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl ester (9CI)

TET Dye Amidite (5'-Tetrachloro-Fluorescein Phosphoramidite)

2',4,7,7'-tetrachloro-6-((6-(((2-cyanoethoxy)(diisopropylamino)phosphanyl)oxy)hexyl)carbamoyl)-3-oxo-3H-spiro [isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethyl propanoate)

6-tet phosphoramidite [5'-tetrachlorofluorescein phosphoramidite

6-(4, 7, 2', 7'-Tetrachloro-3', 6'-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyl)-(N, N-diisopropyl)]-phosphoramidite)

5'-Tetrachlorofluorescein phosphoramidite

6-TET CE Phosphoramidite

877049-90-6

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