2′,7′-Dichlorodihydrofluorescein Diacetate

Product Name: 2′,7′-Dichlorodihydrofluorescein Diacetate
CAS No.: 4091-99-0
Chemical Name: 2′,7′-Dichlorodihydrofluorescein Diacetate
Synonyms: DCFH-DA;DCFH;Dichlorofluorescin diacetate;187263;DCFH | DCFH-DA;H2DCFDA / DCFH-DA;H2DCFDA, 10 mM in DMSO;2'',7''-Dichlorofluorescein diac;2',7'-Dichlorofluorescin diacetat;2'，7'-dichlorofluorescein oxalate
CBNumber: CB8497619
Molecular Formula: C24H16Cl2O7
Formula Weight: 487.29
MOL File: 4091-99-0.mol

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2′,7′-Dichlorodihydrofluorescein Diacetate Property

Melting point:: 209-210 °C(lit.)
Boiling point:: 593.5±50.0 °C(Predicted)
Density: 1.463±0.06 g/cm3(Predicted)
storage temp.: -20°C
solubility: DMF: soluble
form: Crystalline solid
pka: 3.79±0.36(Predicted)
color: White to off-white
Appearance: colorless solid
BRN: 4610273
Stability:: Light Sensitive
InChI: InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChIKey: PXEZTIWVRVSYOK-UHFFFAOYSA-N
SMILES: C(O)(=O)C1=CC=CC=C1C1C2=C(C=C(OC(C)=O)C(Cl)=C2)OC2=C1C=C(Cl)C(OC(C)=O)=C2
EPA Substance Registry System: 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)

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Safety

Hazard Codes: Xi
Risk Statements: 36/37/38
Safety Statements: 22-24/25-26
WGK Germany: 3
F: 10-21
HS Code: 29329990

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: D6883
Product name: 2′,7′-Dichlorofluorescin diacetate
Purity: ≥97%
Packaging: 50mg
Price: $55.1
Updated: 2025/07/31

Sigma-Aldrich

Product number: 287810
Product name: 2?,7?-Dichlorofluorescin Diacetate
Packaging: 100mg
Price: $110
Updated: 2025/07/31

TCI Chemical

Product number: D4409
Product name: 2',7'-Dichlorodihydrofluorescein Diacetate
Packaging: 100MG
Price: $137
Updated: 2025/07/31

Cayman Chemical

Product number: 85155
Product name: 2,7-Dichlorodihydrofluorescein diacetate
Purity: ≥95%
Packaging: 50mg
Price: $33
Updated: 2024/03/01

Cayman Chemical

Product number: 85155
Product name: 2,7-Dichlorodihydrofluorescein diacetate
Purity: ≥95%
Packaging: 100mg
Price: $62
Updated: 2024/03/01

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2′,7′-Dichlorodihydrofluorescein Diacetate Chemical Properties,Usage,Production

Description

DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M⁻¹cm⁻¹).

Chemical Properties

white to yellow-beige or pale pink powder

Uses

Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.

Uses

2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.

Uses

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.

Biochem/physiol Actions

Cell permeable: yes

storage

Store at -20°C

References

[1] N W KOOY H I J A Royall. Oxidation of 2’,7’-dichlorofluorescin by peroxynitrite.[J]. Free Radical Research, 1997, 27 3: 245-254. DOI: 10.3109/10715769709065763
[2] TARABOVá B, LUKE? P, HAMMER M U, et al. Fluorescence measurements of peroxynitrite/peroxynitrous acid in cold air plasma treated aqueous solutions[J]. Physical Chemistry Chemical Physics, 2019, 17: 8883-8896. DOI: 10.1039/c9cp00871c
[3] HYEJIN PARK. Oridonin Enhances Radiation-Induced Cell Death by Promoting DNA Damage in Non-Small Cell Lung Cancer Cells.[J]. International Journal of Molecular Sciences, 2018, 19 8. DOI: 10.3390/ijms19082378
[4] THAMIZHINIYAN VENKATESAN Young K K Young Woong Choi. Effect of an extraction solvent on the antioxidant quality of Pinus densiflora needle extract[J]. Journal of Pharmaceutical Analysis, 2019, 9 3: Pages 193-200. DOI: 10.1016/j.jpha.2019.03.005
[5] LINA DU. Probenecid and N-Acetylcysteine Prevent Loss of Intracellular Glutathione and Inhibit Neuronal Death after Mechanical Stretch Injury In Vitro.[J]. Journal of neurotrauma, 2016, 33 20 1: 1913-1917. DOI: 10.1089/neu.2015.4342
[6] JIN-YI YANG Wei Y Y. Spatiotemporally controlled initiation of Parkin-mediated mitophagy within single cells.[J]. Autophagy, 2011: 1230-1238. DOI: 10.4161/auto.7.10.16626

Abs/Em Maxima

511/533 nm

Excitation / Emission Maximum

492 nm/515 nm

Fluoresene quantum yield

0.76

Extinction Coefficient

118626

CF260

0.17

CF280

0.14

2′,7′-Dichlorodihydrofluorescein Diacetate Preparation Products And Raw materials

Raw materials

Preparation Products

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2′,7′-Dichlorodihydrofluorescein Diacetate Suppliers

Canada 1 China 260 Germany 2 India 4 South Korea 1 Switzerland 2 United Kingdom 2 United States 16 Global 288

Beijing Jin Ming Biotechnology Co., Ltd.

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View Lastest Price from 2′,7′-Dichlorodihydrofluorescein Diacetate manufacturers

Shaanxi Dideu Medichem Co. Ltd

Product: 2′,7′-Dichlorodihydrofluorescein Diacetate 4091-99-0
Price: US $30.00-20.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 200000KG
Release date: 2024-08-06

Career Henan Chemical Co

Product: 2′,7′-Dichlorodihydrofluorescein Diacetate 4091-99-0
Price: US $1.00/KG
Min. Order: 1KG
Purity: 95-99%
Supply Ability: 1ton
Release date: 2019-12-30

4091-99-0, 2′,7′-Dichlorodihydrofluorescein DiacetateRelated Search:

Adenine Sulfate Dihydrate 2',7'-Dichlorofluorescein 2′,7′-Dichlorodihydrofluorescein Diacetate 2',7'-DICHLOROFLUORESCIN DIACETATE 5-(AND-6)-CARBOXY-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE 5-(and-6)-carboxy-2`,7`-dichlorofluorescein diacetate 6-Carboxy-2',7'-dichlorofluorescein 3',6'-Diacetate SucciniMidyl Ester 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 2'',7''-DICHLOROFLUORESCIN DIACETATE 2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE (2',7'-DICHLOROFLUORESCIN DIACETATE; H2DCFDA) 5-CARBOXY-2'',7''-DICHLORODIHYDROFLUORESCEIN DIACETATE, DI(ACETOXYMETHYL ESTER) 5-(AND-6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE, SUCCINIMIDYL ESTER 6-CARBOXY-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE, DI(ACETOXYMETHYL ESTER) 6-CARBOXY-2'',7''-DICHLORODIHYDROFLUORESCIN DIACETATE, DI(ACETOXYMETHYL ESTER) CALCIUM GREEN(TM)-2, AM 5-(AND-6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE 5-(AND-6)-CHLOROMETHYL-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE 2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE, SUCCINIMIDYL ESTER

187263

2,7-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem

2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate

2',7'-DICHLOROFLUORESCIN DIACETATE, FOR FLUORESCENCE

2',7'-Dichlorodihydrofluoresceinediacetate

Dichlorofluorescin diacetate

2'',7''-Dichlorofluorescein diac

2-(2,7-Dichloro-3,6-diacetoxy-9H-xanthen-9-yl)benzoic acid

2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid

2-(3,6-diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

2',7'-DICHLOROFLUORESCIN DI-ACETATE ANALYTICAL GRADE

2′,7′-Dichlorofluorescin diacetate,2′,7′-Dichlorodihydrofluorescein diacetate

2',7'-Dichlorofluorescin diacetat

DCFH

o-(2,7-Dichloro-3,6-dihydroxyxanthen-9-yl)benzoic Acid Diacetate

Dichlorofluorescin diacetate analytical grade

H2DCFDA [2',7'-Dichlorodihydrofluorescein diacetate]

DCFH-DA

H2DCFDA [2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE];DCFH-DA

2-(3,6-Diacetoxy-2,7-dichloro-9H-xanthen-9-yl)

-Dichlorodihydrofluorescein diacetate

2',7'-Dichlorodihydrofluorescein diacetate [2',7'-Dichlorofluorescin diacetate] *CAS 4091-99-0*

2' 7'-DICHLOROFLUORESCIN DIACETATE FOR&

2-[3,6-bis(acetyloxy)-2,7-dichloro-9h-xanthen-9-yl]-benzoicaci

Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-

H2DCFDA[2',7'-DICHLORODIHYDROFLUORESCEINDIACETATChemicalbookE]

DCFH | DCFH-DA

H2DCFDA / DCFH-DA

2′,7′-Dichlorofluorescin diacetate, ≥97%

2,7-Dichlorofluorescein Diacetate (DCFH) extrapure, 97%

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoicaci

2',7'-Dichlorodihydrofluorescein diacetate

2',7'-Dichlorodihydrofluorescein diacetate|||DCFH-DA

2'，7'-dichlorofluorescein oxalate

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DCFH-DA reactive oxygen species ROS fluorescent probe

2'', 7''-Dichlorofluorescin diacetate

H2DCFDA, 10 mM in DMSO

H2-DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] - 100 mg

2'，7'-dichlorofluorescein oxalate

DCFH-DA ROS Fluorescent Probes (Green)

4091-99-0

C24H16Cl2O7