ChemicalBook > CAS DataBase List > 2′,7′-Dichlorodihydrofluorescein Diacetate

2′,7′-Dichlorodihydrofluorescein Diacetate

Product Name
2′,7′-Dichlorodihydrofluorescein Diacetate
CAS No.
4091-99-0
Chemical Name
2′,7′-Dichlorodihydrofluorescein Diacetate
Synonyms
DCFH-DA;DCFH;Dichlorofluorescin diacetate;187263;DCFH | DCFH-DA;H2DCFDA / DCFH-DA;2'',7''-Dichlorofluorescein diac;2',7'-Dichlorofluorescin diacetat;2',7'-dichlorofluorescein oxalate;2',7'-Dichlorofluorescein diacetate
CBNumber
CB8497619
Molecular Formula
C24H16Cl2O7
Formula Weight
487.29
MOL File
4091-99-0.mol
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2′,7′-Dichlorodihydrofluorescein Diacetate Property

Melting point:
209-210 °C(lit.)
Boiling point:
593.5±50.0 °C(Predicted)
Density 
1.463±0.06 g/cm3(Predicted)
storage temp. 
-20°C
solubility 
DMF: soluble
form 
Crystalline solid
pka
3.79±0.36(Predicted)
color 
White to off-white
BRN 
4610273
Stability:
Light Sensitive
EPA Substance Registry System
2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)
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Safety

Hazard Codes 
Xi
Risk Statements 
36/37/38
Safety Statements 
22-24/25-26
WGK Germany 
3
10-21
HS Code 
29329990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H302Harmful if swallowed

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352IF ON SKIN: wash with plenty of soap and water.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P332+P313IF SKIN irritation occurs: Get medical advice/attention.

P337+P313IF eye irritation persists: Get medical advice/attention.

P501Dispose of contents/container to..…

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
D6883
Product name
2′,7′-Dichlorofluorescin diacetate
Purity
≥97%
Packaging
50mg
Price
$53.6
Updated
2024/03/01
Sigma-Aldrich
Product number
287810
Product name
2?,7?-Dichlorofluorescin Diacetate
Packaging
100mg
Price
$107
Updated
2024/03/01
Cayman Chemical
Product number
85155
Product name
2,7-Dichlorodihydrofluorescein diacetate
Purity
≥95%
Packaging
50mg
Price
$33
Updated
2024/03/01
Cayman Chemical
Product number
85155
Product name
2,7-Dichlorodihydrofluorescein diacetate
Purity
≥95%
Packaging
100mg
Price
$62
Updated
2024/03/01
Sigma-Aldrich
Product number
D6883
Product name
2′,7′-Dichlorofluorescin diacetate
Purity
≥97%
Packaging
250mg
Price
$190
Updated
2024/03/01
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2′,7′-Dichlorodihydrofluorescein Diacetate Chemical Properties,Usage,Production

Description

DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1).

Chemical Properties

white to yellow-beige or pale pink powder

Uses

Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.

Uses

2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.

Uses

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.

Biochem/physiol Actions

Cell permeable: yes

storage

Store at -20°C

2′,7′-Dichlorodihydrofluorescein Diacetate Preparation Products And Raw materials

Raw materials

Preparation Products

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2′,7′-Dichlorodihydrofluorescein Diacetate Suppliers

Beijing Jin Ming Biotechnology Co., Ltd.
Tel
010-60605840 18892239720
Fax
010-60605840
Email
psaitong@jm-bio.com
Country
China
ProdList
12306
Advantage
58
Tianjin Kailiqi Biotechnology Co., Ltd.
Tel
15076683720
Fax
022-23754520
Email
klq@cw-bio.com
Country
China
ProdList
4602
Advantage
55
Tianjin Being Technology Co., Ltd.
Tel
18622939839
Fax
QQ:343281693
Email
tjby921@163.com
Country
China
ProdList
510
Advantage
55
Dalian Meilun Biotech Co., Ltd.
Tel
0411-62910999 13889544652
Email
meilunui@163.com
Country
China
ProdList
4727
Advantage
58
Shanghai Qianhao Biotechnology Co., LTD
Tel
13524877553
Email
13524877553@163.com
Country
China
ProdList
32
Advantage
58
Shanghai Boyle Chemical Co., Ltd.
Tel
021-50182298 021-50180596
Fax
+86-21-57758967
Email
sales@boylechem.com
Country
China
ProdList
2208
Advantage
55
J & K SCIENTIFIC LTD.
Tel
010-82848833 400-666-7788
Fax
86-10-82849933
Email
jkinfo@jkchemical.com
Country
China
ProdList
94657
Advantage
76
Beijing HwrkChemical Technology Co., Ltd
Tel
010-89508211 18501085097
Fax
010-89508210
Email
sales.bj@hwrkchemical.com
Country
China
ProdList
8418
Advantage
55
Beijing Ouhe Technology Co., Ltd
Tel
010-82967028 13552068683
Fax
+86-10-82967029
Email
2355560935@qq.com
Country
China
ProdList
12390
Advantage
60
Adamas Reagent, Ltd.
Tel
400-6009262 16621234537
Fax
021-64823266
Email
chenyj@titansci.com
Country
China
ProdList
14103
Advantage
59
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View Lastest Price from 2′,7′-Dichlorodihydrofluorescein Diacetate manufacturers

Shaanxi Dideu Medichem Co. Ltd
Product
2′,7′-Dichlorodihydrofluorescein Diacetate 4091-99-0
Price
US $30.00-20.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
200000KG
Release date
2024-08-06
Hebei Mojin Biotechnology Co., Ltd
Product
2′,7′-Dichlorodihydrofluorescein Diacetate 4091-99-0
Price
US $0.00/KG
Min. Order
1KG
Purity
99%
Supply Ability
50000KG/month
Release date
2023-09-28
Career Henan Chemical Co
Product
2′,7′-Dichlorodihydrofluorescein Diacetate 4091-99-0
Price
US $1.00/KG
Min. Order
1KG
Purity
95-99%
Supply Ability
1ton
Release date
2019-12-30

4091-99-0, 2′,7′-Dichlorodihydrofluorescein DiacetateRelated Search:


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