2',7'-Dichlorofluorescein
Note- Product Name
- 2',7'-Dichlorofluorescein
- CAS No.
- 76-54-0
- Chemical Name
- 2',7'-Dichlorofluorescein
- Synonyms
- 2,7-DICHLOROFLUORESCEIN;DICHLOROFLUORESCEIN;FLUORESCEIN 27;Fluorescein 548;3,6-DICHLOROFLUORAN;2,7-Dichorofluorescin;FLUORESCEIN DICHLORIDE;DICHLOROFLUORESCEIN TS;dcorange25(fluorescein;DICHLORO (R) FLUORESCEIN
- CBNumber
- CB0690319
- Molecular Formula
- C20H10Cl2O5
- Formula Weight
- 401.2
- MOL File
- 76-54-0.mol
2',7'-Dichlorofluorescein Property
- Melting point:
- 280 °C (dec.)(lit.)
- Boiling point:
- 514.77°C (rough estimate)
- Density
- 0.79 g/mL at 20 °C
- refractive index
- 1.5300 (estimate)
- Flash point:
- 12°C (53.6°F)
- storage temp.
- room temp
- solubility
- alcohol: passes test
- form
- crystalline
- pka
- 4.46(at 25℃)
- color
- orange to red-brown, powder
- PH Range
- 4.0 (colorless) - 6.6 (green)
- Water Solubility
- Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water.
- λmax
- 509nm
- BRN
- 58009
- Stability:
- Stable. Incompatible with strong oxidizing agents.
- Major Application
- Electroluminescent devices, sol-gel silica films, integrated circuits, photoresists, lithographic printing plates, recording material, LED devices, photoconductors, Solid-state dye laser, lithographic printing plates, inks, Fluorescent pH sensors, emissive probes, chemotherapeutic treatment of disease, phototherapeutic treatment of disease, photodynamic treatment of disease
- InChIKey
- NZVMJBGGAQSNLI-UHFFFAOYSA-N
- CAS DataBase Reference
- 76-54-0(CAS DataBase Reference)
- EPA Substance Registry System
- Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy- (76-54-0)
Safety
- Hazard Codes
- Xi,F
- Risk Statements
- 36/37/38-36-67-11
- Safety Statements
- 26-36-16
- RIDADR
- UN 1219 3/PG 2
- WGK Germany
- 3
- RTECS
- NT8050000
- TSCA
- Yes
- HS Code
- 3204 19 00
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
- Precautionary statements
-
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
P337+P313IF eye irritation persists: Get medical advice/attention.
N-Bromosuccinimide Price
- Product number
- 35848
- Product name
- 2′,7′-Dichlorofluorescein
- Purity
- BioReagent, suitable for fluorescence, ≥90% (T)
- Packaging
- 5g
- Price
- $96.4
- Updated
- 2024/03/01
- Product number
- 35848
- Product name
- 2′,7′-Dichlorofluorescein
- Purity
- BioReagent, suitable for fluorescence, ≥90% (T)
- Packaging
- 25g
- Price
- $491
- Updated
- 2024/03/01
- Product number
- 1.09676
- Product name
- 2′,7′-Dichlorofluorescein
- Purity
- indicator ACS,Reag. Ph Eur
- Packaging
- 5G
- Price
- $355
- Updated
- 2024/03/01
- Product number
- D0371
- Product name
- 2',7'-Dichlorofluorescein [for Fluorescent indicator]
- Purity
- >95.0%(HPLC)
- Packaging
- 1g
- Price
- $24
- Updated
- 2024/03/01
- Product number
- D0371
- Product name
- 2',7'-Dichlorofluorescein [for Fluorescent indicator]
- Purity
- >95.0%(HPLC)
- Packaging
- 25g
- Price
- $149
- Updated
- 2024/03/01
2',7'-Dichlorofluorescein Chemical Properties,Usage,Production
Note
Hans et al. report that 2',7'-Dichlorofluorescein (DCF) finds legitimate experimental use in the generation of standard curves used to calibrate DCFH2-DA experimental results, post-chromatography estimation of carbohydrates and in indirect fluorimetric detection in open tubular capillary chromatography (OTC-HPLC). However, DCF is inappropriate for demonstrating ROS as it is oxidized to a non-fluorescent compound in the presence of hydroxyl radicles[1].
Chemical Properties
red to yellow solid
Uses
Widely used probe, e.g. for determination of carbohydrates by fluorescence densitometry after TLC and determination of H2O2 by chemiluminescence. Fluorescent additives used in indirect fluorimetric detection in OTC-HPLC.
Uses
2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.
A new fluorescent sensor for Cd2+ and its application in living cells imaging. Tetrahedron Lett. 2015, 56 (11), 1322-1327.
Colorimetric and fluorogenic signaling of fluoride ions by thiophosphinated dichlorofluorescein. Dyes Pigm. 2015, 112, 170-175.
Uses
2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.
Definition
2',7'-dichlorofluorescein is a member of 2-benzofurans. It has a role as a fluorochrome. It derives from a fluorescin.
Application
2',7'-dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.
Biological Activity
2',7'-dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).
Biochem/physiol Actions
2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).
References
[1] Chander Hans. “2’,7’-Dichlorofluorescein (DCF) or 2’,7’-dichlorodihydrofluorescein diacetate (DCFH2-DA) to measure reactive oxygen species in erythrocytes.” Biomedicine & Pharmacotherapy (2021): 111512.
2',7'-Dichlorofluorescein Preparation Products And Raw materials
Raw materials
Preparation Products
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View Lastest Price from 2',7'-Dichlorofluorescein manufacturers
- Product
- dichlorofluorescein 76-54-0
- Price
- US $5.00-2.00/kg
- Min. Order
- 1kg
- Purity
- 99%
- Supply Ability
- g-kg-tons, free sample is available
- Release date
- 2024-04-16
- Product
- dichlorofluorescein 76-54-0
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 50000KG/month
- Release date
- 2023-10-16
- Product
- dichlorofluorescein 76-54-0
- Price
- US $0.00/KG
- Min. Order
- 1KG
- Purity
- 99%
- Supply Ability
- 500000kg
- Release date
- 2023-03-21