2',7'-Dichlorofluorescein

ChemicalBook > CAS DataBase List > 2',7'-Dichlorofluorescein

2',7'-Dichlorofluorescein

Note

Product Name: 2',7'-Dichlorofluorescein
CAS No.: 76-54-0
Chemical Name: 2',7'-Dichlorofluorescein
Synonyms: 2,7-DICHLOROFLUORESCEIN;DICHLOROFLUORESCEIN;FLUORESCEIN 27;Fluorescein 548;3,6-DICHLOROFLUORAN;2,7-Dichorofluorescin;FLUORESCEIN DICHLORIDE;DICHLOROFLUORESCEIN TS;dcorange25(fluorescein;DICHLORO (R) FLUORESCEIN
CBNumber: CB0690319
Molecular Formula: C20H10Cl2O5
Formula Weight: 401.2
MOL File: 76-54-0.mol

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2',7'-Dichlorofluorescein Property

Melting point:: 280 °C (dec.)(lit.)
Boiling point:: 514.77°C (rough estimate)
Density: 0.79 g/mL at 20 °C
refractive index: 1.5300 (estimate)
Flash point:: 12°C (53.6°F)
storage temp.: room temp
solubility: alcohol: passes test
form: crystalline
pka: 4.46(at 25℃)
color: orange to red-brown, powder
PH Range: 4.0 (colorless) - 6.6 (green)
Water Solubility: Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water.
λmax: 509nm
BRN: 58009
Stability:: Stable. Incompatible with strong oxidizing agents.
Major Application: Electroluminescent devices, sol-gel silica films, integrated circuits, photoresists, lithographic printing plates, recording material, LED devices, photoconductors, Solid-state dye laser, lithographic printing plates, inks, Fluorescent pH sensors, emissive probes, chemotherapeutic treatment of disease, phototherapeutic treatment of disease, photodynamic treatment of disease
InChIKey: NZVMJBGGAQSNLI-UHFFFAOYSA-N
CAS DataBase Reference: 76-54-0(CAS DataBase Reference)
EPA Substance Registry System: Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 2',7'-dichloro-3',6'-dihydroxy- (76-54-0)

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Safety

Hazard Codes: Xi,F
Risk Statements: 36/37/38-36-67-11
Safety Statements: 26-36-16
RIDADR: UN 1219 3/PG 2
WGK Germany: 3
RTECS: NT8050000
TSCA: Yes
HS Code: 3204 19 00

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Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Warning

Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

Precautionary statements

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.

P337+P313IF eye irritation persists: Get medical advice/attention.

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N-Bromosuccinimide Price

Sigma-Aldrich

Product number: 35848
Product name: 2′,7′-Dichlorofluorescein
Purity: BioReagent, suitable for fluorescence, ≥90% (T)
Packaging: 5g
Price: $96.4
Updated: 2024/03/01

Sigma-Aldrich

Product number: 35848
Product name: 2′,7′-Dichlorofluorescein
Purity: BioReagent, suitable for fluorescence, ≥90% (T)
Packaging: 25g
Price: $491
Updated: 2024/03/01

Sigma-Aldrich

Product number: 1.09676
Product name: 2′,7′-Dichlorofluorescein
Purity: indicator ACS,Reag. Ph Eur
Packaging: 5G
Price: $355
Updated: 2024/03/01

TCI Chemical

Product number: D0371
Product name: 2',7'-Dichlorofluorescein [for Fluorescent indicator]
Purity: >95.0%(HPLC)
Packaging: 1g
Price: $24
Updated: 2024/03/01

TCI Chemical

Product number: D0371
Product name: 2',7'-Dichlorofluorescein [for Fluorescent indicator]
Purity: >95.0%(HPLC)
Packaging: 25g
Price: $149
Updated: 2024/03/01

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2',7'-Dichlorofluorescein Chemical Properties,Usage,Production

Note

Hans et al. report that 2',7'-Dichlorofluorescein (DCF) finds legitimate experimental use in the generation of standard curves used to calibrate DCFH2-DA experimental results, post-chromatography estimation of carbohydrates and in indirect fluorimetric detection in open tubular capillary chromatography (OTC-HPLC). However, DCF is inappropriate for demonstrating ROS as it is oxidized to a non-fluorescent compound in the presence of hydroxyl radicles[1].

Chemical Properties

red to yellow solid

Uses

Widely used probe, e.g. for determination of carbohydrates by fluorescence densitometry after TLC and determination of H2O2 by chemiluminescence. Fluorescent additives used in indirect fluorimetric detection in OTC-HPLC.

Uses

2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.
A new fluorescent sensor for Cd2+ and its application in living cells imaging. Tetrahedron Lett. 2015, 56 (11), 1322-1327.
Colorimetric and fluorogenic signaling of fluoride ions by thiophosphinated dichlorofluorescein. Dyes Pigm. 2015, 112, 170-175.

Uses

Definition

2',7'-dichlorofluorescein is a member of 2-benzofurans. It has a role as a fluorochrome. It derives from a fluorescin.

Application

2',7'-dichlorofluorescein has been used as an oxidation-sensitive fluorescent probe to measure ROS (reactive oxygen species) formation in cells.

Biological Activity

2',7'-dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).

Biochem/physiol Actions

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe. 2′7′-dichlorofluorescein diacetate is oxidized to 2′7′-dichlorofluorescein in the presence of ROS (reactive oxygen species).

References

[1] Chander Hans. “2’,7’-Dichlorofluorescein (DCF) or 2’,7’-dichlorodihydrofluorescein diacetate (DCFH2-DA) to measure reactive oxygen species in erythrocytes.” Biomedicine & Pharmacotherapy (2021): 111512.

2',7'-Dichlorofluorescein Preparation Products And Raw materials

Raw materials

Preparation Products

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View Lastest Price from 2',7'-Dichlorofluorescein manufacturers

Henan Fengda Chemical Co., Ltd

Product: dichlorofluorescein 76-54-0
Price: US $5.00-2.00/kg
Min. Order: 1kg
Purity: 99%
Supply Ability: g-kg-tons, free sample is available
Release date: 2024-04-16

Hebei Mojin Biotechnology Co., Ltd

Product: dichlorofluorescein 76-54-0
Price: US $0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 50000KG/month
Release date: 2023-10-16

Hebei Guanlang Biotechnology Co., Ltd.

Product: dichlorofluorescein 76-54-0
Price: US $0.00/KG
Min. Order: 1KG
Purity: 99%
Supply Ability: 500000kg
Release date: 2023-03-21

76-54-0, 2',7'-DichlorofluoresceinRelated Search:

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