Chemical Name Alkaloids Detection method
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1-Deoxynojirimycin

Chemical Name Alkaloids Detection method
Product Name
1-Deoxynojirimycin
CAS No.
19130-96-2
Chemical Name
1-Deoxynojirimycin
Synonyms
DNJ;moranoline;DEOXYNOJIRIMYCIN;Sepetaprost;Duvoglustat;DNM;S-GI;moranolin;bay-h5595;uvoglusta
CBNumber
CB9128150
Molecular Formula
C6H13NO4
Formula Weight
163.17
MOL File
19130-96-2.mol
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1-Deoxynojirimycin Property

Melting point:
195-196°C
Boiling point:
361.1±42.0 °C(Predicted)
Density 
1.456±0.06 g/cm3(Predicted)
vapor pressure 
0Pa at 25℃
RTECS 
TN4350300
storage temp. 
Keep in dark place,Inert atmosphere,Store in freezer, under -20°C
solubility 
Soluble in Water up to 25 mg/ml).
pka
13.77±0.70(Predicted)
form 
Powder
color 
White
Water Solubility 
Soluble in water, dimethyl sulfoxide and methanol.
BRN 
3588039
Stability:
Stable for 2 years from date of purchase as supplied. Solutions in distilled water may be stored at -20° for up to 3 months.
LogP
-1.8
CAS DataBase Reference
19130-96-2(CAS DataBase Reference)
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Safety

Safety Statements 
24/25
WGK Germany 
1
HS Code 
29329990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Warning
Hazard statements

H315Causes skin irritation

H319Causes serious eye irritation

H335May cause respiratory irritation

Precautionary statements

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
08012
Product name
1-Deoxynojirimycin
Purity
analytical standard
Packaging
10mg
Price
$623
Updated
2024/03/01
Sigma-Aldrich
Product number
SAB5300111
Product name
Monoclonal Anti-DNM1 antibody produced in mouse
Purity
clone 3G4B6, ascites fluid
Packaging
100μL
Price
$508
Updated
2022/05/15
Alfa Aesar
Product number
J62602
Product name
(+)-1-Deoxynojirimycin
Packaging
100mg
Price
$563
Updated
2024/03/01
Cayman Chemical
Product number
21500
Product name
1-Deoxynojirimycin
Purity
≥95%
Packaging
5mg
Price
$62
Updated
2024/03/01
Cayman Chemical
Product number
21500
Product name
1-Deoxynojirimycin
Purity
≥95%
Packaging
10mg
Price
$109
Updated
2024/03/01
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1-Deoxynojirimycin Chemical Properties,Usage,Production

Chemical Name

(2R,3R,4R,5S)-2-Hydroxymethyl-piperidine-3,4,5-triol

Alkaloids

1-deoxynojirimycin  is referred DNJ for short  , it is an alkaloid extracted from the bark of mulberry leaves and roots, but it also exists in other plants and microorganisms. This product is an effective α-glucosidase inhibitor, it has significant hypoglycemic effect. After 1-deoxynojirimycin goes into the human body, it can inhibit sucrose, maltase, α-glucosidase enzyme, α-amylase decomposing  starch, sugar in the human body , thereby it can block the body's absorption of sugar, inhibiting blood sugar rising to achieve the effect of prevention and treatment of diabetes, the use of it does not cause changes in diet . In addition, DNJ can inhibit glucose modification process of HIV tunica glycoprotein , at the same time, the accumulation of immature glycoproteins may inhibit cell fusion, viral and host cell receptor can combine,which causes  syncytia formation to inhibit the replication of  MoLV ,then the virus activity is inhibited.
Nojirimycin is first discovered from Streptomyces, and natural DNJ is first isolated from the bark of mulberry root. In plants, from mulberry, dayflower, hyacinth and Adenophora plants, DNJ has been isolated and identified ,  DNJ has the highest content in the mulberry and because of mulberry varieties, medicinal parts, seasonal climate, geography, soil, leaf position, different developmental stages  and other factors , there is a big difference. In a microorganism, from a variety of Streptomyces and Bacillus,DNJ is isolated ,it is also found that two kinds of endophytes separated from Mulberry including Stenotrophomonas oligotrophic Pseudomonas and Micrococcus can produce DNJ,fermentation conditions of a variety of  microbial production of DNJ are studied. In insects, in addition to silkworm rich in DNJ , single or oligophagous insects with eating mulberry leaves habit including wild silkworm, mulberry geometrid, Diaphania pyloalis Walker , mulberry white capterpillar are also rich in DNJ , DNJ in insects bodies are from the food , content of DNJ in Bombyx bodies is different due to the different varieties of silkworm, developmental stages, tissues and organs as well as feed and other factors, with the silkworm age of progress ,there is the existence of cyclical changes in absorption and accumulation and excretion of DNJ. Now DNJ biosynthetic pathways in Streptomyces, Bacillus and Commelina bodies are explored and it is found that  synthesis of DNJ has different mechanisms in different species . In addition, three main synthesis methods of 1-deoxynojirimycin are proven , some of the synthetic derivatives of DNJ have been used clinically.
Recent studies show that the active ingredient of mulberry DNJ (l-deoxynojirimycin), only exists in mulberry leaves , by blocking the α-glucosidase enzymes to hinder sugar  becoming to glucose, mulberry leaf extract can inhibit intestinal glucose absorption. This can suppress the blood sugar level and blood pressure rising , and it can have good inhibitory effect on variability of imidazopyridine, benzopyrene and other carcinogenic substances,it has anti-cancer effect, at the same time ,mulberry leaf extract can reduce cholesterol, and improve liver function and eliminate constipation and so on.

Detection method

After extraction, 1-Deoxynojirimycin (DNJ) contents can be determined by HPLC using various detectors such as fluorescent, evaporative light-scattering detectors, pulsed amperometric, and Mass spectrometers. Since the DNJ molecule does not contain a chromophore, the quantification of DNJ through spectral analysis needs derivatization, i.e., an analytical approach for the quantification of nitrogen-containing compounds. To quantify and resolve structural information of DNJ by GC-MS, Bajpai and Rao used trimethylsilyl (TMS) derivatization.

Description

Deoxynojirimycin (19130-96-2) inhibits α-glucosidase I and II.1,2 Inhibits human immunodeficiency virus envelope glycoprotein-mediated membrane fusion at the CXCR4 binding step.3 May be used to produce an affinity ligand for purifying glucosidase 1.4 Deoxynojirimycin was used to inhibit ER glucosidases I and II allowing for the discovery of a second mechanism for deglucosylation of N-linked oligosaccharides in PhaR1.7, a mouse lymphoma cell line.5

Chemical Properties

White Crystalline Solid

Occurrence

1-Deoxynojirimycin (DNJ), a product of fermentation, exists in mulberry leaves and Commelina communis (dayflower) as well as is isolated from several bacterial strains such as Bacillus and Streptomyces species. Mulberry, a common deciduous plant, belongs to the genus Morus (Moraceae family). Numerous researchers have reported the DJN contents in different parts and various varieties of mulberry. Various mulberry varieties contain DNJ contents ranging between 0.68 and 2.78 mg/gm, while this fraction varies between 1.57 and 3.48 mg/gm in different Chinese mulberry leaves. Moreover, mulberry shoots contain the highest contents of DNJ, followed by young mulberry leaves. Mature leaves of mulberry contain the least content of DNJ. All the mulberry leave groups showed a 1–2-fold increase in DNJ contents compared to unfermented mulberry leaf powder extract (UFMLE). Additionally, fermented mulberry leaf powder extract exhibited higher α-glucosidase activity than UFMLE. DNJ-rich food products can be prepared by fermenting mulberry leaves using the above-mentioned fermenting agents[6].

Uses

Deoxynojirimycin inhibits mammalian glucosidase 1. As well, it inhibits intestinal and lysosmal alpha-glucosidases, beta-glucosidase from sweet almonds, pancreatic alpha-amylase and amyloglucosidase.

Uses

An inhibitor of α-glucosidase I and II

Uses

An alpha-glucosidase inhibitor. Interferes with normal processing of N-linked glycoproteins.(+)-1-Deoxynojirimycin acts as an inhibitor of alfa-glucosidase I and II and maltase-glucoamylase. It also inhibits mammalian glucosidase, intestinal and lysosmal, beta-glucosidase from sweet almonds, pancreatic alfa-amylase and amyloglucosidase. Further, it serves as a enzyme enhancer for the treatment of Fabry and Pompe disease.

Definition

ChEBI: An optically active form of 2-(hydroxymethyl)piperidine-3,4,5-triol having 2R,3R,4R,5S-configuration.

Biological Activity

Inhibitor of glucosidase I (K i = 2.1 mM) and II (K i = 7 mM).

storage

+4°C

References

1) Fuhrmann et al. (1985), Inhibitors of oligosaccharide processing; Biochim. Biophys, Acta, 825 95 2) Hughs and Rudge (1994), Deoxynojirimycin: synthesis and biological activity; Nat. Prod. Rep., 11 135 3) Papandreou et al. (2002), The alpha-glucosidase inhibitor 1-deoxynojirimycin blocks human immunodeficiency virus envelope glycoprotein-mediated membrane fusion at the CXCR4 binding step; Mol. Pharmacol., 61 186 4) Hettkamp et al. (1984), Purification by affinity chromatography of glucosidase I, an endoplasmic reticulum hydrolase involved in the processing of asparagine-linked oligosaccharides; Eur. J. Biochem., 142 85 5) Suh et al. (1992), Identification of a novel mechanism for the removal of glucose residues from high mannose-type oligosaccharides; J. Biol. Chem., 267 21671.
[6] Kuo Gao. “1-Deoxynojirimycin: Occurrence, Extraction, Chemistry, Oral Pharmacokinetics, Biological Activities and In Silico Target Fishing.” Molecules 21 1 (2016).

1-Deoxynojirimycin Preparation Products And Raw materials

Raw materials

Preparation Products

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1-Deoxynojirimycin Suppliers

ShangHai YuanYe Biotechnology Co., Ltd.
Tel
021-61312847 13636370518
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021-55068248
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shyysw007@163.com
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China
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4940
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Chengdu Forest Science and Technology Development Co., Ltd.
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18030648795 18030648795
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028-61777050
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sales@cdforestchem.com
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China
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Chengdu Biopurify Phytochemicals Ltd.
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+86-028-82633397 18982077548
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+86-28-82633165
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cwb1@biopurify.cn
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J & K SCIENTIFIC LTD.
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010-82848833 400-666-7788
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86-10-82849933
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jkinfo@jkchemical.com
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China
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3B Pharmachem (Wuhan) International Co.,Ltd.
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821-50328103-801 18930552037
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86-21-50328109
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3bsc@sina.com
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Chembest Research Laboratories Limited
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+86-21-20908456
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021-58180499
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sales@BioChemBest.com
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China
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Energy Chemical
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021-021-58432009 400-005-6266
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021-58436166
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sales8178@energy-chemical.com
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Jia Xing Isenchem Co.,Ltd
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0573-85285100 18627885956
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0573-85285100
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isenchem@163.com
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China
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Shanghai Hanhong Scientific Co.,Ltd.
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021-54306202 13764082696
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info@hanhongsci.com
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China
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Chemsky(shanghai)International Co.,Ltd.
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021-50135380
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shchemsky@sina.com
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China
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View Lastest Price from 1-Deoxynojirimycin manufacturers

Changsha Staherb Natural Ingredients Co., Ltd.
Product
1-Deoxynojirimycin; 1-DNJ 19130-96-2
Price
US $0.00/KG
Min. Order
1KG
Purity
≥98% HPLC
Supply Ability
1000KG
Release date
2022-09-20
Hebei Zhuanglai Chemical Trading Co Ltd
Product
Deoxynojirimycin 19130-96-2
Price
US $150.00/kg
Min. Order
1kg
Purity
99%
Supply Ability
500kg
Release date
2024-12-10
WUHAN FORTUNA CHEMICAL CO., LTD
Product
1-Deoxynojirimycin 19130-96-2
Price
US $0.00/KG
Min. Order
1KG
Purity
98%min
Supply Ability
30tons/month
Release date
2023-02-15

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