ChemicalBook > CAS DataBase List > 3,5-Dinitrosalicylic acid

3,5-Dinitrosalicylic acid

Product Name
3,5-Dinitrosalicylic acid
CAS No.
609-99-4
Chemical Name
3,5-Dinitrosalicylic acid
Synonyms
3,5-dinitrosalicylic;2-HYDROXY-3,5-DINITROBENZOIC ACID;3,5-DINITRO-2-HYDROXYBENZOIC ACID;NSC 181;RARECHEM AL BE 0171;3,5-Dinitro salicyli;Dinitro salicylic ac;LABOTEST-BB LT00060163;5-Dinitrosalicylic acid;3,5-Dinitrosalicyls?ure
CBNumber
CB9172047
Molecular Formula
C7H4N2O7
Formula Weight
228.12
MOL File
609-99-4.mol
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3,5-Dinitrosalicylic acid Property

Melting point:
168-172 °C (lit.)
Boiling point:
369.91°C (rough estimate)
Density 
1.7914 (rough estimate)
bulk density
400kg/m3
refractive index 
1.5300 (estimate)
storage temp. 
room temp
solubility 
H2O: soluble50mg/mL
pka
1.57±0.10(Predicted)
form 
Powder
color 
Yellow
PH
1.3-1.8 (20°C, 10g/L in H2O)
PH Range
1.3-1.8 (10g/L @ 20°C)
Water Solubility 
soluble
BRN 
2220661
Stability:
Stable. Incompatible with strong oxidizing agents, strong bases, strong acids.
LogP
0.12 at 23℃ and pH2.6-2.8
CAS DataBase Reference
609-99-4(CAS DataBase Reference)
NIST Chemistry Reference
3,5-Dinitrosalicylic acid(609-99-4)
EPA Substance Registry System
Benzoic acid, 2-hydroxy-3,5-dinitro- (609-99-4)
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Safety

Hazard Codes 
Xn,Xi
Risk Statements 
22-37/38-36/37/38
Safety Statements 
22-24/25-36-26
RIDADR 
2811
WGK Germany 
3
RTECS 
VO2965000
Hazard Note 
Irritant
TSCA 
Yes
HazardClass 
6.1(b)
PackingGroup 
III
HS Code 
29182990
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Hazard and Precautionary Statements (GHS)

Symbol(GHS)
Signal word
Danger
Hazard statements

H228Flammable solid

H302Harmful if swallowed

Precautionary statements

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P240Ground/bond container and receiving equipment.

P241Use explosion-proof electrical/ventilating/lighting/…/equipment.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P301+P312IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell.

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N-Bromosuccinimide Price

Sigma-Aldrich
Product number
128848
Product name
3,5-Dinitrosalicylic acid
Purity
98%
Packaging
5g
Price
$19.7
Updated
2025/07/31
Sigma-Aldrich
Product number
128848
Product name
3,5-Dinitrosalicylic acid
Purity
98%
Packaging
100g
Price
$96.8
Updated
2025/07/31
TCI Chemical
Product number
D0850
Product name
3,5-Dinitrosalicylic Acid
Purity
>98.0%(GC)(T)
Packaging
25g
Price
$35
Updated
2025/07/31
TCI Chemical
Product number
D0850
Product name
3,5-Dinitrosalicylic Acid
Purity
>98.0%(GC)(T)
Packaging
100g
Price
$96
Updated
2025/07/31
Sigma-Aldrich
Product number
D0550
Product name
3,5-Dinitrosalicylic acid
Purity
used in colorimetric determination of reducing sugars
Packaging
10g
Price
$36.8
Updated
2025/07/31
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3,5-Dinitrosalicylic acid Chemical Properties,Usage,Production

Chemical Properties

3,5-Dinitrosalicylic acid appears as yellow, flaky crystals with a melting point of 173–174 °C. It is soluble in alcohol, benzene, and ether but only slightly soluble in water.

Uses

3,5-Dinitrosalicylic acid is a salicylic acid derivative used in colorimeteric determination of reducing sugars.

Uses

3,5-Dinitrosalicylic acid is used in the quantification of reducing sugars by colorimetry. It is utilized for the analysis of glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. It is also used to detect reducing substances in urine and for quantifying carbohydrate level in blood. It acts as an important raw material and an intermediate in the production of organic chemicals such as agrochemicals, organic synthesis, pharmaceuticals and dyes.

Uses

3,5-Dinitrosalicylic acid was used as a reagent for the preparation of oxazolines from amino alcohols and for the spectrophotometric determination of ampicillin. It was also used to measure the effects of silver nanoparticles on the membrane leakage of the reducing sugars.

Definition

ChEBI: A monohydroxybenzoic acid consisting of 2-hydroxybenzoic acid having nitro substituents at the 3- and 5-positions. It is used in colorimetric testing for the presence of free carbonyl groups (C2O) in reducing sugars.

Preparation

3,5-Dinitrosalicylic acid is prepared by the nitration of salicylic acid. Salicylic acid is added to a mixed acid solution and nitrated at 15–25°C. The reaction mixture is then slowly poured into ice water, yielding a yellow solid precipitate. After filtration and washing with water, the pure product is obtained.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Purification Methods

Crystallise the acid from H2O. [Beilstein 10 IV 270.]

3,5-Dinitrosalicylic acid Preparation Products And Raw materials

Raw materials

Nitrification

Preparation Products

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3,5-Dinitrosalicylic acid Suppliers

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View Lastest Price from 3,5-Dinitrosalicylic acid manufacturers

Shaanxi Dideu New Materials Co. Ltd
Product
3,5-Dinitrosalicylic acid 609-99-4
Price
US $0.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
1000kgs
Release date
2025-11-18
WUHAN FORTUNA CHEMICAL CO., LTD
Product
3,5-Dinitrosalicylic acid 609-99-4
Price
US $0.00/KG
Min. Order
1KG
Purity
98%min
Supply Ability
30tons/month
Release date
2023-01-16
Henan Aochuang Chemical Co.,Ltd.
Product
3,5-Dinitrosalicylic acid 609-99-4
Price
US $0.00-0.00/kg
Min. Order
1kg
Purity
98%
Supply Ability
1Ton
Release date
2022-10-19

609-99-4, 3,5-Dinitrosalicylic acidRelated Search:

4-Methylsalicylic acid Folic acid 3-Nitrosalicylic acid Ethyl 2-(Chlorosulfonyl)acetate 1,3-DINITRONAPHTHALENE 5-Nitrosalicylic acid Citric acid Ascoric Acid Methyl 3,5-dinitrosalicylate 3,5-DINITROSALICYLIC ACID SODIUM,SODIUM 3,5-DINITROSALICYLIC ACID,3,5-DINITROSALICYLIC ACID, MONOSODIUM SALT 3,5-Dinitrosalicylic acid 2-(5-nitrofurfurylidene) hydrazide,3,5-Dinitrosalicylic acid N'-(5-nitrofurfurylidene) hydrazide 2,4-Dinitrophenylhydrazine 2,5-DINITROPHENOL 1,3-Dinitrobenzene 3,5-Dinitrobenzoic acid Salicylic acid 3,5-Dinitrosalicylic acid 3,5-DINITROSALICYLIC HYDRAZIDE

  • 2-hydroxy-3,5-dinitro-benzoicaci
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  • 3,5-DINITROSALICYLIC ACID / 3,5-DINITRO-2-HYDROXYBENZOIC ACID
  • 3,5-Dinitrosalicylic acid, 97+%
  • 3,5-Dinitrosalicylic Acid Solution [for Spray]
  • 3,5-Dinitrosalicylic acid, 98+%
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  • 3-5,DinitroSalicylicAcidForSynthesis
  • 3-5,DinitroSalicylicAcidForSynthesis-(3-5,Dinitro-2-HydroxybenzoicAcid)
  • 3,5-DinitrosalicylicAcid99%
  • 3,5-DinitrosalicyclicAcidForSynthesis
  • 3-5,DinitroSalicylicAcidGr
  • 3-5, DINTRO SALICYLIC ACID FOR SYNTHESIS
  • 3,5-Dinitro salicyli
  • 3,5-Dinitrosalyicylic acid
  • 3,5-DINITROSALICYLIC ACID extrapure AR
  • 3,5-Dinitrosalicylic acid, 98% 100GR
  • Dinitro salicylic ac
  • NSC 181
  • 3,5-Dinitrosalicylic Acid Solution
  • 3,5-Dinitrosalicylic Acid &gt
  • 5-Dinitrosalicylic acid
  • 3,5-Dinitrosalicylic acid, ≥98% (HPLC and titration)
  • 3,5-Dinitrosalicyclic acid 98%
  • 3,5-Dinitrosalicylic acid, GR 99%+
  • 3,5-Dinitrosalicyls?ure
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  • 3,5-Dinitrosalicylic Acid 97% For Synthesis
  • 609-99-4
  • 609-99-6
  • O2N2C6H22OHCO2H
  • C7H4N2O7
  • C7H2N2O7
  • Organic Building Blocks
  • C7
  • Carbonyl Compounds
  • Carboxylic Acids
  • Building Blocks
  • Aromatics
  • Miscellaneous Reagents
  • Aromatic Carboxylic Acids, Amides, Anilides, Anhydrides & Salts
  • Organic acids
  • bc0001
  • HL00006