RABBIT MONOCLONAL ANTI-FLAGCLONE SIGI-25
Immunogen Background- Product Name
- RABBIT MONOCLONAL ANTI-FLAGCLONE SIGI-25
- Chemical Name
- RABBIT MONOCLONAL ANTI-FLAGCLONE SIGI-25
- Synonyms
- ANTI-ECS;ANTI-DDDDK;flag antibody;Flag? Affinity resin;ANTI-FLAG? M2 Affinity Gel;Anti-FLAG? M2 Magnetic Beads;FLAG? Immunoprecipitation Kit;FLAG? magnetic affinity resin;ANTI-ENTEROKINASE CLEAVAGE SITE;FLAG? resin for high throughput
- CBNumber
- CB9198368
- Formula Weight
- 0
- MOL File
- Mol file
RABBIT MONOCLONAL ANTI-FLAGCLONE SIGI-25 Property
- storage temp.
- -20°C
- form
- buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate,)
- biological source
- rabbit
Safety
- Hazard Codes
- Xi,Xn
- Risk Statements
- 36/38-43-52/53-22-21/22-36-41
- Safety Statements
- 26-36/37-61-39
- WGK Germany
- 3
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H317May cause an allergic skin reaction
H319Causes serious eye irritation
H411Toxic to aquatic life with long lasting effects
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P264Wash hands thoroughly after handling.
P264Wash skin thouroughly after handling.
P273Avoid release to the environment.
P280Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352IF ON SKIN: wash with plenty of soap and water.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- F3165
- Product name
- Monoclonal ANTI-FLAG<SUP>®</SUP> M2 antibody produced in mouse
- Purity
- clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium
- Packaging
- 0.2 mg
- Price
- $567
- Updated
- 2025/07/31
- Product number
- F3165
- Product name
- Monoclonal ANTI-FLAG<SUP>®</SUP> M2 antibody produced in mouse
- Purity
- clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium
- Packaging
- 1 mg
- Price
- $1037.4
- Updated
- 2025/07/31
- Product number
- F1804
- Product name
- Monoclonal ANTI-FLAG<SUP>®</SUP> M2 antibody produced in mouse
- Purity
- 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM s
- Packaging
- 50 μg
- Price
- $142
- Updated
- 2025/07/31
- Product number
- F3165
- Product name
- Monoclonal ANTI-FLAG<SUP>®</SUP> M2 antibody produced in mouse
- Purity
- clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium
- Packaging
- 5 mg
- Price
- $3300
- Updated
- 2025/07/31
- Product number
- F1804
- Product name
- Monoclonal ANTI-FLAG<SUP>®</SUP> M2 antibody produced in mouse
- Purity
- 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM s
- Packaging
- 200 μg
- Price
- $492.44
- Updated
- 2025/07/31
RABBIT MONOCLONAL ANTI-FLAGCLONE SIGI-25 Chemical Properties,Usage,Production
Uses
Monoclonal ANTI-FLAG? M2-Alkaline Phosphatase antibody produced in mouse has been used:
- in direct tissue blot immunoassay of sweet orange petioles samples
- in screening internalization of delta opioid receptor
- for screening cell-free protein expression using ELISA
Uses
Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000
Browse additional application references in our FLAG? Literature portal.
Uses
ANTI-FLAG? High Sensitivity, M2 coated 96-well plates is used for Enzyme-linked immunosorbent assays. A convenient, ready to use, platform for the capture and detection of FLAG? fusion proteins. The ANTI-FLAG? M2 antibody is covalently attached to the surface through the Fc portion and can detect 1 ng FLAG fusion protein/well with a capacity of up to 300ng/well. Suitable for screening for expression, study of protein:protein interactions and ELISA assays. Manufactured under ISO 9002 in Sigma′s GMP facility, ANTI-FLAG?P2983 high sensitivity M2 coated multiwell plates utilize a flat bottom, polystyrene baseplate.
Browse additional application references in our FLAG? Literature portal.
General Description
Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is produced in mouse and covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications.
Biochem/physiol Actions
Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.
Immunogen
Flag-conjugated proteins.
Background
A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. FLAG is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody. denoted as M1. binds the peptide in the presence of bivalent metal cations. preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free Flag Peptide.
The FLAG peptide purifies and detects recombinant fusion proteins. FLAG peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. Flag peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
RABBIT MONOCLONAL ANTI-FLAGCLONE SIGI-25 Preparation Products And Raw materials
Raw materials
Preparation Products
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