Hygromycin B Property

Melting point:

160-180°C

alpha 

D20 +20.2° (c = 1 in water)

Boiling point:

608.13°C (rough estimate)

Density 

1.4252 (rough estimate)

refractive index 

1.6300 (estimate)

storage temp. 

2-8°C

solubility 

H₂O: 50 mg/mL As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.

form 

powder

pka

7.1, 8.8(at 25℃)

color 

faintly brown to brown

PH

pH (25g/l, 25℃ ) : 9.5～12.5

Water Solubility 

Miscible with water, methanol, buffer solution and ethanol. Immiscible with less polar solvents.

Merck 

14,4852

BRN 

6755837

Stability:

Hygroscopic

EPA Substance Registry System

Hygromycin B (31282-04-9)

Safety

Hazard Codes 

T+,Xn,T

Risk Statements 

26/27/28-41-42/43-40-36-23/24/25

Safety Statements 

23-26-28-36/37/39-45-22-36-36/37

RIDADR 

UN 3462 6.1/PG 1

WGK Germany 

3

RTECS 

WK2130000

F 

19

HazardClass 

6.1

PackingGroup 

I

HS Code 

29419059

Toxicity

mmo-esc 250 mg/L AACHAX 18,798,80

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Precautionary statements

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P284Wear respiratory protection.

Hygromycin B Chemical Properties,Usage,Production

Description

Hygromycin B is an aminoglycoside antibiotic that has been found in S. hygroscopicus. It is active against E. coli (MIC = 150 μg/ml) and cytotoxic to both prokaryotic and eukaryotic cells via inhibition of protein synthesis. Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing the E. coli hygromycin resistance genes hyg or hph.

Chemical Properties

White to off-white lyophilized powder

Uses

antibacterial, anthelmintic; LD50 ip (rat) 63mg/Kg

Uses

Hygromycin B, a selective antibiotic that is effective on most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome

Uses

Hygromycin B is an aminoglycoside and a selective antibiotic agent for the hph gene. Hygromycin B inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S rib osome

Uses

Hygromycin B is a trisaccharide aminoglycoside with a terminal cyclitol produced by Streptomyces hygroscopicus, reported by researchers at Eli Lily in the early 1950s. Hygromycin B belongs to the destomycin family of aminoglycosides which contain a unique cyclic structure linking the terminal saccharide. Hygromycin B exhibits broad spectrum antibiotic and anthelmintic activity, and is used as a feed additive for swine and poultry. Hygromycin B inhibits polypeptide synthesis by binding peptidyl-tRNA and preventing translocation by Elongation Factor 2.

Application

Hygromycin B was found in the culture broth of Streptomyces hygroscopicus by Lilly Research Laboratories in 1953, as a mixture with hygromycin A. It showed activity against a variety of gram-positive and gram-negative bacteria as well as fungi. Hygromycin B has been used for therapy of helminthic infections in swine and poultry.

General Description

Amorphous solid or tan powder.

Air & Water Reactions

Water soluble.

Reactivity Profile

Weakly basic.

Fire Hazard

Flash point data for Hygromycin B are not available; however Hygromycin B is probably combustible.

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Safety Profile

Poison by intraperitoneal route. Mutation data reported. When heated to decomposition it emits toxic fumes of Nox

storage

+4°C

References

[1] brodersen de, clemons wm jr, carter ap, et al. the structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin b on the 30s ribosomal subunit. cell, 2000, 103 (7): 1143-1154.
[2] ganoza mc, kiel mc. a ribosomal atpase is a target for hygromycin b inhibition on escherichia coli ribosomes. antimicrob agents chemother, 2001, 45 (10): 2813-2819.