PAF Acetylhydrolase Assay Kit
- Product Name
- PAF Acetylhydrolase Assay Kit
- Chemical Name
- PAF Acetylhydrolase Assay Kit
- Synonyms
- PAF Acetylhydrolase Assay Kit
- CBNumber
- CB92591936
- Formula Weight
- 0
- MOL File
- Mol file
N-Bromosuccinimide Price
- Product number
- 760901
- Product name
- PAF Acetylhydrolase Assay Kit
- Packaging
- 96Well
- Price
- $244
- Updated
- 2024/03/01
PAF Acetylhydrolase Assay Kit Chemical Properties,Usage,Production
Description
Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.1 The Cayman Chemical PAF acetylhydrolase assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH.2 Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5'-dithio-bis(2-nitrobenzoic acid) (DTNB; Ellman’s reagent). The dynamic range of the assay is only limited by the accuracy of the absorbance measurement. Most plate readers are linear to an absorbance of 1.2. The detection range of the assay is from 0.02 to 0.2 µmol/min/ml of PAF-AH activity which is equivalent to an absorbance increase of 0.01 to 0.1 per minute. Each kit contains assay buffer, DTNB, 2-thio PAF (substrate), human PAF-AH standard, a 96 well plate, and complete instructions.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
PAF Acetylhydrolase Assay Kit Preparation Products And Raw materials
Raw materials
Preparation Products
PAF Acetylhydrolase Assay Kit Suppliers
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