Bromocresol green Property

Melting point:

225 °C (dec.) (lit.)

Density 

0.79 g/mL at 20 °C

Flash point:

12 °C

storage temp. 

no restrictions.

solubility 

Solubility Sparingly soluble in water; soluble in ethanol, ether, ethyl acetate

form 

Powder/Solid

pka

4.7(at 25℃)

Boiling point:

626.0±55.0 °C(Predicted)

color 

Pale orange

PH Range

3.8(YELLOW)-5.4(BLUE)

PH

3.8～5.4

Water Solubility 

slightly soluble

λmax

423nm, 444nm, 617nm

Merck 

14,1386

BRN 

372527

Stability:

Stable. Incompatible with strong oxidizing agents.

Major Application

Sol-gel matrix, fuel cells, sensors, display device, inks, paints, one-steptester for ammonia, cosmet-tester for ammonia, packaging system, textiles, cleansing products, detergents, cosmetics, diapers, identifying fresh and stale rice, monitoring fish spoilage, detecting carbohydrates, lacting acid bacteria, treating neoplasms, cancer, vaginal infection test method, drugs, dental materials

LogP

7.800 (est)

CAS DataBase Reference

76-60-8(CAS DataBase Reference)

EPA Substance Registry System

Bromcresol green (76-60-8)

Safety

Hazard Codes 

F,Xi,Xn

Risk Statements 

11-36-67-36/37/38-20/21/22

Safety Statements 

7-16-24/25-26-37/39-36

RIDADR 

UN 1219 3/PG 2

WGK Germany 

3

RTECS 

SJ7456000

Autoignition Temperature

399 °C

TSCA 

Yes

HazardClass 

3

PackingGroup 

II

HS Code 

29349990

Hazard and Precautionary Statements (GHS)

Symbol(GHS)

Signal word

Danger

Hazard statements

H225Highly Flammable liquid and vapour

H319Causes serious eye irritation

H340May cause genetic defects

H360May damage fertility or the unborn child

H372Causes damage to organs through prolonged or repeated exposure

H373May cause damage to organs through prolonged or repeated exposure

Precautionary statements

P201Obtain special instructions before use.

P202Do not handle until all safety precautions have been read and understood.

P210Keep away from heat/sparks/open flames/hot surfaces. — No smoking.

P233Keep container tightly closed.

P240Ground/bond container and receiving equipment.

P260Do not breathe dust/fume/gas/mist/vapours/spray.

P264Wash hands thoroughly after handling.

P264Wash skin thouroughly after handling.

P270Do not eat, drink or smoke when using this product.

P271Use only outdoors or in a well-ventilated area.

P280Wear protective gloves/protective clothing/eye protection/face protection.

P303+P361+P353IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower.

P308+P313IF exposed or concerned: Get medical advice/attention.

P403+P233Store in a well-ventilated place. Keep container tightly closed.

P405Store locked up.

P501Dispose of contents/container to..…

Bromocresol green Chemical Properties,Usage,Production

Chemical Properties

tan to yellow crystals

Uses

Bromocresol Green is a pH sensitive indicator used in the growth medium for microorganisms and titrations. It is useful in various colorimetric detection studies and as an actively tracking dye for DNA agarose gel electrophoresis. It acts as an inhibitor of prostaglandin E2 transport protein. It is also used in TLC for visualizing compounds containing functional groups having pKa less than 5.

Uses

Bromocresol Green (BCG) is a pH sensitive triphenylmethane dye useful in a variety of colorimetric detection technologies. BCG is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations and in charge-transfer complexation processes. In TLC BCG is used for visualisation of the compounds with functional groups whose pKa is below 5.0.

Uses

As indicator, pH 3.8 yellow; pH 5.4 blue-green.

Definition

ChEBI: Bromocresolgreen is a member of benzofurans.

Hazard

Moderately toxic.

Purification Methods

Crystallise the dye from glacial acetic acid or dissolve it in aqueous 5% NaHCO3 solution and precipitate it from the hot solution by dropwise addition of aqueous HCl. Repeat this until the UV/VIS-extinction did not increase 423nm. It is an indicator: at pH 3.81 at max (yellow) and pH 5.4 (blue-green). [Beilstein 19/3 V 460.]