4-Amidinophenylmethanesulfonyl fluoride hydrochloride Chemical Properties

Melting point:

~205 °C (dec.)

storage temp. 

-20°C

solubility 

H₂O: 50 mM Stable when aliquoted at −20°C. Half-life = 6 minutes in pH 7.0 buffer systems.

form 

White solid

color 

white

Water Solubility 

water: 25mg/mL

BRN 

9083148

InChIKey

KHLLRHIUKOJXLL-UHFFFAOYSA-N

CAS DataBase Reference

74938-88-8(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xn

Risk Statements 

20/21/22-36/37/38

Safety Statements 

22-24/25-36-26

WGK Germany 

3

F 

10-21

HS Code 

29252900

MSDS

• Language:English Provider:SigmaAldrich

4-Amidinophenylmethanesulfonyl fluoride hydrochloride Usage And Synthesis

Description

p-APMSF is an irreversible inhibitor of serine proteases with K_i values of 1.02, 1.18, 1.5, and 1.54 μM for bovine trypsin, human thrombin, bovine plasmin, and bovine Factor Xa, respectively. It is selective for these proteases over bovine chymotrypsin and acetylcholinesterase.

Uses

p-APMSF is an irreversible inhibitor of serinase. p-APMSF has substrate specificity for positively charged side chains of amino acids, lysine, or arginine. p-APMSF can be used to study the irreversible inhibition of trypsin and thrombin [1].

Biochem/physiol Actions

Irreversible inhibitor of serine proteases with lysine or arginine substrate specificities. Effective concentration 10-100 μM. Frequently used to characterize newly discovered proteases.

References

[1] Laura R, et al. (p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor of serine proteases. Biochemistry. 1980 Oct 14;19(21):4859-64. DOI:10.1021/bi00562a024

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