P-nitrophenyl butyrate Chemical Properties

Boiling point:

184-185 °C

Density 

1.19 g/mL at 20 °C(lit.)

storage temp. 

-20°C

solubility 

chloroform: 0.1 g/mL, clear, faintly yellow

form 

A neat oil

BRN 

1879729

InChIKey

DVDUMIQZEUTAGK-UHFFFAOYSA-N

Safety Information

Hazard Codes 

Xi

Risk Statements 

43

Safety Statements 

36/37

WGK Germany 

3

HS Code 

29156000

MSDS

• Language:English Provider:SigmaAldrich

P-nitrophenyl butyrate Usage And Synthesis

Description

P-nitrophenyl butyrate also kown as 4-nitrophenyl butyrate, is a probe for lipase activity. It is hydrolyzed by lipase in the water ambient condition, giving rise to p-nitrophenol and butyric acid. P-nitrophenol actually occurs in the anionic form, p-nitrophenolate, giving a yellowish coloration and absorbing light at 410 nm in an alkaline buffer such as Tris-HCl pH 7.5.

Uses

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.

Definition

ChEBI: P-nitrophenyl butyrate is a butyrate ester resulting from the formal condensation of the hydroxy group of 4-nitrophenol with the carboxy group of butyric acid. It is a butyrate ester and a C-nitro compound. It is functionally related to a 4-nitrophenol.

References

[1] POHANKA M. Biosensors and Bioassays Based on Lipases, Principles and Applications, a Review.[J]. Molecules, 2019. DOI:10.3390/molecules24030616.
[2] WENTING SHANG. Enzymatic hydrolysis of p-nitrophenyl butyrate in CO2-based micelle systems[J]. Journal of chemical technology and biotechnology, 2013, 89 9: 1324-1329. DOI:10.1002/jctb.4246.
[3] SIGRUN H. STERRI Frode F Bj?rn A Johnsen. A radiochemical assay method for carboxylesterase, and comparison of enzyme activity towards the substrates methyl [1-14C] butyrate and 4-nitrophenyl butyrate[J]. Biochemical pharmacology, 1985, 34 15: Pages 2779-2785. DOI:10.1016/0006-2952(85)90579-9.
[4] BISHNU PRASAD PANDEY. Bergenia pacumbis from Nepal, an astonishing enzymes inhibitor.[J]. BMC Complementary Medicine and Therapies, 2020: 198. DOI:10.1186/s12906-020-02989-2.

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