ANTI-PCNA Chemical Properties

storage temp. 

-20°C

form 

buffered aqueous solution

biological source

rabbit

Safety Information

Hazard Codes 

Xn

Risk Statements 

21/22

Safety Statements 

36/37

WGK Germany 

WGK 3

Storage Class

10 - Combustible liquids

ANTI-PCNA Usage And Synthesis

Uses

Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used in:

• enzyme linked immuno sorbent assay (ELISA)
• immunoblotting
• immunohistology
• immunoprecipitation
• flow cytometry

General Description

PCNA (proliferating cell nuclear antigen) is a member of the structurally and functionally conserved family of DNA sliding clamps (β clamps). They exist as ring-shaped complexes- homotrimers in eukaryotes, having pseudohexameric symmetry. A monomer of PCNA is composed of two similar globular domains, connected by an interdomain connecting loop, which is a long, and probably a flexible loop. These monomers organize themselves in a head-to-tail manner to form a ring, which has an inner positively charged surface composed of α helices and an outer surface of β sheets.

Biochem/physiol Actions

Proliferating cell nuclear antigen (PCNA) is essential for DNA replication during S-phase. It is essential for cellular DNA synthesis. PCNA is required for leading strand synthesis in the SV40 system where it acts as an auxiliary protein for polymerase.