5-FAM Me-tetrazine
5-FAM Me-tetrazine Basic information
- Product Name:
- 5-FAM Me-tetrazine
- Synonyms:
-
- 5-FAM Me-tetrazine
- 6-Methyl-Tetrazine-5-FAM
- Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxamide, 3',6'-dihydroxy-N-[[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]methyl]-3-oxo-
- CAS:
- 1619222-85-3
- MF:
- C31H21N5O6
- MW:
- 559.53
- Mol File:
- 1619222-85-3.mol
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5-FAM Me-tetrazine Chemical Properties
- Density
- 1.61±0.1 g/cm3(Predicted)
- solubility
- Soluble in DMF, DMSO
- pka
- 9.32±0.20(Predicted)
- Appearance
- yellow solid
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5-FAM Me-tetrazine Usage And Synthesis
Description
Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.
Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.
Uses
6-Methyl-Tetrazine-5-FAM, is a Fluorescent dye, that can be used for various labeling applications in chemistry. (Abs/Em = 492/517 nm)
Abs/Em Maxima
492/517 nm
Fluoresene quantum yield
0.93
Extinction Coefficient
74000
CF260
0.22
CF280
0.17
5-FAM Me-tetrazineSupplier
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