Cy5.5-SE Chemical Properties

solubility 

Easily soluble in organic solvents such as DMSO and DMF, very soluble in water

form 

Solid

color 

Light blue to blue

Appearance

Dark blue solid

ex/em

678/706 nm (PBS buffer)

ε(extinction coefficient)

211000 L⋅mol−1⋅cm−1

Φ(quantum yield)

0.21

InChIKey

DOMDXTIMIZCSNC-UHFFFAOYSA-N

SMILES

CC1(C(N(CCCCCC(=O)ON2C(CCC2=O)=O)C2C=CC3=C(C=C(S(O)(=O)=O)C=C3C1=2)S(O)(=O)=O)=CC=CC=CC1C(C2C3=CC(S(O)(=O)=O)=CC(S([O-])(=O)=O)=C3C=CC=2[N+]=1CC)(C)C)C

Cy5.5-SE Usage And Synthesis

Chemical Properties

Appearance: Dark blue solid
ex/em: 678/706 nm (PBS buffer)
Extinction coefficient (ε): 211000 Lmol1cm1
Quantum yield (Φ): 0.21

Uses

Cy5.5-SE (Cyanine5.5 NHS ester) is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis[1]. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance[2]. Storage: protect from light.

in vivo

References

[1] Zhu S, et al. Visualizing cancer and response to therapy in vivo using Cy5.5-labeled factor VIIa and anti-tissue factor antibody. J Drug Target. 2015 Apr;23(3):257-65. DOI:10.3109/1061186X.2014.988217
[2] Ptaszek M. Rational design of fluorophores for in vivo applications. Prog Mol Biol Transl Sci. 2013;113:59-108. DOI:10.1016/B978-0-12-386932-6.00003-X
[3] Shindy, H. A. (2017). Fundamentals in the chemistry of cyanine dyes: A review. Dyes and Pigments, 145, 505–513. doi:10.1016/j.dyepig.2017.06.029
[4] Jiang L, et al. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin for MR and fluorescence imaging of glioma in rats. Biomaterials. 2013 Oct;34(30):7418-28. DOI:10.1016/j.biomaterials.2013.05.078
[5] Lim B, et al. A Unique Recombinant Fluoroprobe Targeting Activated Platelets Allows In Vivo Detection of Arterial Thrombosis and Pulmonary Embolism Using a Novel Three-Dimensional Fluorescence Emission Computed Tomography (FLECT) Technology. Theranostics. 2017 Feb 26;7(5):1047-1061. DOI:10.7150/thno.18099

Cy5.5-SE(442912-55-2)Related Product Information

• Cyanine5.5 maleimide
• Cyanine 5.5 hydrazide [equivalent to Cy5.5 hydrazide]
• Cy5.5-conjugated Goat anti-human IgG
• 1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-, chloride (1:1)
• Sulfo-Cy5.5 amine