FLUORESCEIN-12-DUTP Chemical Properties

storage temp. 

-20°C

solubility 

Water: soluble

form 

solution

FLUORESCEIN-12-DUTP Usage And Synthesis

Uses

Fluorescein-12-dUTP is used as a substrate for:

• terminal transferase
• DNA polymerase I (holoenzyme and Klenow fragment),
• Taq DNA polymerase
• reverse transcriptase (e.g., from AMV and M-MuLV).

Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

References

[1] J KOCH. Fast one-step procedure for the detection of nucleic acids in situ by primer-induced sequence-specific labeling with fluorescein-12-dUTP.[J]. Cytogenetics and cell genetics, 1992, 60 1: 1-3. DOI: 10.1159/000133281

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