Basic information Safety Supplier Related

FLUORESCEIN-12-DUTP

Basic information Safety Supplier Related

FLUORESCEIN-12-DUTP Basic information

Product Name:
FLUORESCEIN-12-DUTP
Synonyms:
  • CHROMATIDE(TM) FLUORESCEIN-12-DUTP
  • FLUORESCEIN-12-DUTP
  • FLUOR-12-DUTP
  • FLUORESCEIN-6-CARBOXAMINOCAPROYL-[5-[3-AMINOALLYL]-2'-DEOXYURIDINE-5'-TRIPHOSPHATE]
MF:
C38H41N4O19P3
MW:
950.67
Mol File:
Mol File
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FLUORESCEIN-12-DUTP Chemical Properties

storage temp. 
-20°C
form 
solution
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FLUORESCEIN-12-DUTP Usage And Synthesis

Uses

Fluorescein-12-dUTP is used as a substrate for:

  • terminal transferase
  • DNA polymerase I (holoenzyme and Klenow fragment),
  • Taq DNA polymerase
  • reverse transcriptase (e.g., from AMV and M-MuLV).
Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

FLUORESCEIN-12-DUTPSupplier

Sigma-Aldrich
Tel
021-61415566 800-8193336
Email
orderCN@merckgroup.com
BOC Sciences
Tel
16314854226
Email
inquiry@bocsci.com
BOC Sciences
Tel
+1-631-485-4226
Email
inquiry@bocsci.com
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FLUORESCEIN-12-DUTP()Related Product Information