Biotin-16-UTP Chemical Properties

storage temp. 

-20°C

form 

solution

color 

colorless

Water Solubility 

water: miscible

Biotin-16-UTP Usage And Synthesis

Description

Biotin-16-UTP is a biotin-labeled UTP used for in vitro transcription to prepare RNA probes[1]. Biotin-16-UTP is an active substrate for RNA polymerase and can be incorporated by a variety of RNA polymerases (including SP6, T3, and T7 RNA polymerases) to form labeled RNA[2, 3]. Biotin-16-UTP-labeled RNA can be detected by reporter molecules linked to streptavidin, avidin, or anti-biotin antibodies[4, 5]. Biotin-16-UTP can be used for molecular hybridization and RNA-protein interaction studies[6].

Uses

Biotin-labeled RNA can be used as a hybridization probe for:

• Northern blots
• Southern blots
• Plaque or colony lifts
• RNase protection experiments
• In situ hybridization
• Microarray hybridization

The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

General Description

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

References

[1] McKenney K M, Connacher R P, Dunshee E B, et al. Chemi-Northern: a versatile chemiluminescent northern blot method for analysis and quantitation of RNA molecules[J]. RNA, 2024, 30(4): 448-462.
[2] Chien H, Alston C I, Dix R D. Suppressor of cytokine signaling 1 (SOCS1) and SOCS3 are stimulated within the eye during experimental murine cytomegalovirus retinitis in mice with retrovirus-induced immunosuppression[J]. Journal of Virology, 2018, 92(18): 10.1128/jvi. 00526-18.
[3] MINGMING CHEN. A novel lncRNA promotes myogenesis of bovine skeletal muscle satellite cells via PFN1-RhoA/Rac1[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021, 25 13: 5988-6005. DOI:10.1111/jcmm.16427.
[4] Ritchey B, Hai Q, Han J, et al. Genetic variant in 3’untranslated region of the mouse pycard gene regulates inflammasome activity[J]. Elife, 2021, 10: e68203.
[5] Bratthauer G L. The avidin–biotin complex (ABC) method and other avidin–biotin binding methods[J]. Immunocytochemical methods and protocols, 2010: 257-270.
[6] Siprashvili Z, Webster D E, Kretz M, et al. Identification of proteins binding coding and non-coding human RNAs using protein microarrays[J]. BMC genomics, 2012, 13: 1-13.

Biotin-16-UTP()Related Product Information

• 5-Me-UTP,100mM Sodium Salt Solution
• T7 Co-Transcription RNA Kit (N1-Me-pUTP)
• Cy5 Luciferase mRNA(5-oME-UTP)
• UTP
• Pseudouridine 5’-Triphosphate
• Cy3-UTP 10mM Sodium Solution
• EPO mRNA(N1-Me-pUTP)
• OVA mRNA(5-oME-UTP)
• Luciferase mRNA(N1-Me-pUTP)
• Cy5 eGFP mRNA(5-oME-UTP)