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2-((E)-3-[1-(3-[[3-(DIMETHYL(3-[4-[(E)-3-(3-METHYL-1,3-BENZOTHIAZOL-3-IUM-2-YL)-2-PROPENYLIDENE]-1(4H)-PYRIDINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-3-METHYL-1,3-BENZOTHIAZOL-3-IUM TETRAIODIDE

Basic information Safety Supplier Related

2-((E)-3-[1-(3-[[3-(DIMETHYL(3-[4-[(E)-3-(3-METHYL-1,3-BENZOTHIAZOL-3-IUM-2-YL)-2-PROPENYLIDENE]-1(4H)-PYRIDINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-3-METHYL-1,3-BENZOTHIAZOL-3-IUM TETRAIODIDE Basic information

Product Name:
2-((E)-3-[1-(3-[[3-(DIMETHYL(3-[4-[(E)-3-(3-METHYL-1,3-BENZOTHIAZOL-3-IUM-2-YL)-2-PROPENYLIDENE]-1(4H)-PYRIDINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-3-METHYL-1,3-BENZOTHIAZOL-3-IUM TETRAIODIDE
Synonyms:
  • 2-((E)-3-[1-(3-[[3-(DIMETHYL(3-[4-[(E)-3-(3-METHYL-1,3-BENZOTHIAZOL-3-IUM-2-YL)-2-PROPENYLIDENE]-1(4H)-PYRIDINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-3-METHYL-1,3-BENZOTHIAZOL-3-IUM TETRAIODIDE
  • BOBO 3
  • BOBO-3 IODIDE
  • BENZOTHIAZOLIUM, 2,2'-[1,3-PROPANEDIYLBIS[(DIMETHYLIMINIO)-3,1-PROPANEDIYL-1(4H)-PYRIDINYL-4-YLIDENE-1-PROPENYL-3-YLIDENE]]BIS[3-METHYL-, TETRAIODIDE
  • Benzothiazolium, 2,2 0 -[1,3-propanediyl- bis[(dimethyliminio)-3,1-propanediyl-1(4 h )-pyridinyl- 4-ylidene-1-propen-1-yl-3-ylidene]]bis[3-methyl, tetra- iodide
  • Bobo 3, Bibo 3 Iodide
CAS:
169454-17-5
MF:
C45H58I4N6S2
MW:
1254.73
Product Categories:
  • Cyanine
Mol File:
169454-17-5.mol
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2-((E)-3-[1-(3-[[3-(DIMETHYL(3-[4-[(E)-3-(3-METHYL-1,3-BENZOTHIAZOL-3-IUM-2-YL)-2-PROPENYLIDENE]-1(4H)-PYRIDINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-3-METHYL-1,3-BENZOTHIAZOL-3-IUM TETRAIODIDE Chemical Properties

solubility 
Soluble in dimethyl sulfoxide
form 
powder
color 
Yellow-brown
Appearance
purple solution
ex/em
570/602 nm
ε(extinction coefficient)
147800 cm−1M−1
Φ(quantum yield)
0.39
λmax
570 nm (H2O/DNA)
Major Application
Semiconductor nanocrystal
Biological Applications
Nucleic acid hybridization; DNA fingerprinting; DNA sequencing; detecting nucleic acids,cells,pathogens; counting embryoblasts
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2-((E)-3-[1-(3-[[3-(DIMETHYL(3-[4-[(E)-3-(3-METHYL-1,3-BENZOTHIAZOL-3-IUM-2-YL)-2-PROPENYLIDENE]-1(4H)-PYRIDINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-3-METHYL-1,3-BENZOTHIAZOL-3-IUM TETRAIODIDE Usage And Synthesis

Description

BODi-3 (also known as BOBO®-3) is a orange fluorescent carbocyanine dimeric dye. BODi-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits a multiple fluorescence enhancement upon binding to dsDNA.


The bright fluorescence signal and low background make BODi-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.


The dye is used to image cell nuclei, label bacteria and sperms, perform nucleic acid detection and amplification, and as a temperature sensor.

Chemical Properties

ex/em: 570/602 nm
Extinction coefficient (ε): 147800 cm??1M??1
Quantum yield (Φ): 0.39

Definition

ChEBI: BoBo-3 is an organic iodide salt and a cyanine dye. It has a role as a fluorochrome. It contains a BoBo-3(4+).

Abs/Em Maxima

565 (complex)/606 (complex) nm

2-((E)-3-[1-(3-[[3-(DIMETHYL(3-[4-[(E)-3-(3-METHYL-1,3-BENZOTHIAZOL-3-IUM-2-YL)-2-PROPENYLIDENE]-1(4H)-PYRIDINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-PYRIDINYLIDENE]-1-PROPENYL)-3-METHYL-1,3-BENZOTHIAZOL-3-IUM TETRAIODIDESupplier

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