CASPASE-3 Chemical Properties

Melting point:

127 °C

storage temp. 

−70°C

solubility 

PBS: soluble

form 

buffered aqueous glycerol solution

Safety Information

WGK Germany 

1

MSDS

• Language:English Provider:SigmaAldrich

CASPASE-3 Usage And Synthesis

Uses

Caspase 3 human has been used to digest human r-vimentin. It has also been used in caspase-3 inhibition assay and protease inhibition assay.

General Description

Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.

Biochem/physiol Actions

Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase?3 is considered to be an effector caspase, activating pro-caspase?6 and pro-caspase?9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.