YOYO 3 Chemical Properties

Melting point:

>250 ℃

solubility 

Soluble in dimethyl sulfoxide

form 

powder

color 

Orange-red

Appearance

dark violet solution

λmax

612 nm

ex/em

612/631 nm

ε(extinction coefficient)

167000 cm−1M−1

Φ(quantum yield)

0.15

Major Application

Analyzing polymers

Biological Applications

Nucleic acid hybridization; detecting nucleic acids,ells,pathogens; counting embryoblasts; monitoring cell cycle kinetics

YOYO 3 Usage And Synthesis

Description

YODi-3 (Oxazole Red Homodimer, also known as YOYO^®-3) is a red fluorescent carbocyanine dimeric dye. YODi-3 is a cell-impermeant nucleic acid stain that is nonfluorescent in the absence of nucleic acids but exhibits significant fluorescence enhancement upon binding to DNA.

The bright fluorescence signal and low background make YODi-3 ideal for staining nucleic acids on microarrays, as well as for nuclear and chromosome counterstaining in multicolor fluorescence labeling experiments.

Simultaneous labeling with cell-impermeant YODi-3 and cell-permeable LUCS 13 or Annexin V-AF488 can be used to assess cell viability and apoptosis.

Chemical Properties

ex/em: 612/631 nm
Extinction coefficient (ε): 167000 cm??1M??1
Quantum yield (Φ): 0.15

Uses

YOYO-3 is a cell-impermeant dye with far-red fluorescence used as nulear counterstain and dead cell indicator. It generates bright fluorescence upon binding to DNA.

Abs/Em Maxima

612/631 nm

Excitation / Emission Maximum

612 nm/631 nm

YOYO 3(156312-20-8)Related Product Information

• YO-PRO 3
• YO-PRO 1
• 2-([1-(3-[[3-(DIMETHYL(3-[4-[(E)-(3-METHYL-1,3-BENZOXAZOL-3-IUM-2-YL)METHYLIDENE]-1(4H)-QUINOLINYL]PROPYL)AMMONIO)PROPYL](DIMETHYL)AMMONIO]PROPYL)-4(1H)-QUINOLINYLIDENE]METHYL)-3-METHYL-1,3-BENZOXAZOL-3-IUM TETRAIODIDE
• 1-ETHYL-4-METHYLQUINOLINIUM IODIDE
• 4-methyl-1-propylquinolinium iodide
• YOYO 3
• N,N'-BIS(3-AMINOPROPYL)-1,3-PROPANEDIAMINE
• POPO 3