FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Chemical Properties

Melting point:

>200℃

Boiling point:

1063.2±65.0 °C(Predicted)

Density 

1.47

storage temp. 

−20°C

solubility 

DMSO: soluble

pka

1.92±0.50(Predicted)

form 

Solid

color 

Brown to reddish brown

Appearance

orange-red powder

λmax

456nm

Biological Applications

Calcium indicator

InChIKey

QOMNQGZXFYNBNG-UHFFFAOYSA-N

SMILES

C(OCOC(C)=O)(=O)CN(C1=CC=C(C2C3=C(C=C(OCOC(C)=O)C(F)=C3)OC3C=2C=C(F)C(=O)C=3)C=C1OCCOC1=CC(C)=CC=C1N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

Safety Information

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

3

F 

10-21

HS Code 

32049010

FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5 Usage And Synthesis

Description

Fluo-4 AM is a cell-permeable Ca²⁺-indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon Ca²⁺-binding (excitation/emission λ at 494/506 nm). Fluo-4 AM is used for visualization and measurement of intracellular Ca²⁺. It is well suited for fluorometric and imaging applications such as microscopy, flow cytometry, spectrofluorometry, and fluorometric high-throughput microplate screening assays [1].

Fluo-4 AM is similar in structure and spectral properties to the widely used Ca²⁺-indicator, Fluo-3, but it has certain advantages over Fluo-3, such as brighter fluorescence emission, high rate of cell permeation, and a K_d for Ca²⁺ in buffer of 345 nM. Because of its higher fluorescence emission intensity, Fluo-4 AM can be used at lower intracellular concentrations, making its use less toxic for live cells.

As Fluo-4 AM does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. In vivo cell imaging with Fluo-4 AM is usually performed within one or two hours after loading, but the dye can be re-loaded to cells if it is needed. Fluo-4 AM can also be fixed in situ by EDC/EDAC for downstream immunofluorescence studies.

Fluo-4 AM has low solubility in the water. It is recommended to prepare 1 mM stock solution in labeling grade DMSO prior to cell loading. Use the final concentration of 1-5 μM and incubation at 37 °C for 15-60 min as a start point of your protocol.

Uses

Fluorescent Ca2+ indicator dye; cell-permeable AM ester, insensitive to magnesium.

Uses

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator. Displays high fluorescence emission. Excitation/emission λ 494/516 nm.

storage

Store at -20°C

Abs/Em Maxima

494/518 nm

Excitation / Emission Maximum

494 nm/506 nm

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