AC-YVAD-AFC Chemical Properties

Melting point:

>190°C (dec.)

Boiling point:

1084.6±65.0 °C(Predicted)

Density 

1.416±0.06 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

DMSO (Slightly), Methanol (Silghtly)

form 

powder

pka

4.09±0.10(Predicted)

color 

white

Safety Information

WGK Germany 

3

MSDS

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AC-YVAD-AFC Usage And Synthesis

Description

Ac-YVAD-AFC is a substrate whose amino acids YVAD have been shown to be a preferred cleavage site for caspase-1 and -4. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.

Uses

N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-trifluoromethylcoumarin is a substrate whose amino acids are shown as a preferred cleavage site for Caspase-1 and 4. Useful for caspase research.

Definition

ChEBI: Ac-Tyr-Val-Ala-Asp-7-amino-4-trifluoromethylcoumarin is a tetrapeptide obtained by formal condensation of the C-terminal carboxy group of Ac-Tyr-Val-Ala-Asp with the amino group of 7-amino-4-trifluoromethylcoumarin. It has a role as a protease inhibitor. It is a tetrapeptide, a member of acetamides, a member of coumarins and an organofluorine compound. It is functionally related to a 7-amino-4-(trifluoromethyl)coumarin.

Biological Activity

Fluorogenic peptide substrate for caspase-1 (ICE).

AC-YVAD-AFC(219137-85-6)Related Product Information

• AC-YVAD-CHO
• AC-YVAD-AMC
• AC-YVAD-AFC