4,5-DIAMINOFLUORESCEIN Chemical Properties

storage temp. 

2-8°C

form 

powder

color 

Brown to black

Safety Information

Hazard Codes 

Xn,Xi

Risk Statements 

22-36/37/38

Safety Statements 

23-26-36

WGK Germany 

3

F 

8-10-23

MSDS

• Language:English Provider:SigmaAldrich

4,5-DIAMINOFLUORESCEIN Usage And Synthesis

Description

DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM.

Uses

DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity.

Uses

Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells.

References

[1] H KOJIMA. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.[J]. Chemical & pharmaceutical bulletin, 1998, 46 2: 373-375. DOI: 10.1248/cpb.46.373
[2] NAOKI NAKATSUBO. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins[J]. FEBS Letters, 1998, 427 2: 263-266. DOI: 10.1016/s0014-5793(98)00440-2

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