DAF-FM Chemical Properties

Melting point:

265 ℃

storage temp. 

-20°C

solubility 

DMSO: >1mg/mL

form 

Solid

pka

8.19 ± 0.20, most acidic, temperature: 25 °C; 4.23 ± 0.20, most basic(at 25℃)

color 

clear colorless to yellow

λmax

487 nm, 495 nm

Biological Applications

Nitric oxide indicator; detecting microorganisms; measuring Ga i-coupled or Gao-coupled receptors activity

Safety Information

Hazard Codes 

Xn

Risk Statements 

22

WGK Germany 

3

DAF-FM Usage And Synthesis

Uses

DAF-FM is important reagent for quantitating low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotrizole.

Uses

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Uses

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.

Biochem/physiol Actions

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.