C6-NBD-CERAMIDE Chemical Properties

Melting point:

85-88 °C

Density 

1.153±0.06 g/cm3(Predicted)

storage temp. 

−20°C

solubility 

Soluble in DMSO (35 mg/mL)

pka

13.54±0.20(Predicted)

form 

solid

color 

Orange

Stability:

Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.

Safety Information

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

C6-NBD-CERAMIDE Usage And Synthesis

Description

C6 NBD ceramide is a biologically active fluorescent analog of short chain, membrane-permeable ceramides. It is as effective as C6 ceramide in the inhibition of viral glycoprotein transport through the Golgi. C6 NBD ceramide has been used as a fluorescent substrate for the activity of UDP-glucose:ceramide glucosyltransferase and to demonstrate the translocation of glucocerebroside and sphingomyelin from the Golgi to the plasma membrane.

Chemical Properties

Rust or Dark Yellow Solid

Uses

A vital fluoresent stain for the Golgi apparatus.

Definition

ChEBI: N-{6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoyl}sphingosine is an N-acylsphingosine that is sphingosine with the amino nitrogen converted into a 6-{[N-(7-nitrobenzo-2,1,3-oxadiazol-4-yl)amino]}hexananamido group. It has a role as a fluorescent probe.

References

Chazotte (2012),?Labeling Golgi with Fluorescent Ceramides;?Cold Spring Harb. Protoc. (8):pdb.prot070599 van Meer et al. (1987),?Sorting of sphingolipids in epithelial (Madin-Darby canine kidney) cells; J. Cell Biol.?105 1623 Don and Rosen (2008),?A fluorescent plate reader assay for ceramide kinase; Anal. Biochem.?375 265 Nikolova-Karakashian (2021),?Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics; Methods Mol. Biol.?2187 113

C6-NBD-CERAMIDE(94885-02-6)Related Product Information

• 3-[(Aminocarbonyl)amino]-5-(3-fluorophenyl)-N-(3S)-3-piperidinyl-2-Thiophenecarboxamide
• 9-Cyclopentyl-2-[[2-methoxy-4-[(1-methylpiperidin-4-yl)oxy]-phenyl]amino]-7-methyl-7,9-dihydro-8H-purin-8-one
• Pomalidomide
• Acalabrutinib
• NBD-H
• Ceramide
• C6 CERAMIDE
• C6-NBD-CERAMIDE
• D-ERYTHRO-SPHINGOSINE