DI-4-ANEPPS Chemical Properties

Melting point:

122–124℃

storage temp. 

2-8°C

solubility 

DMF: soluble

form 

powder

color 

orange to red

Appearance

Orange solid

ex/em

483/686 nm (artificial membrane)

λmax

495 nm

ε(extinction coefficient)

≥38000 at 493-499nm in methanol

Biological Applications

Measuring membrane potential; preventing arrhythmias; probes for Na,K-ATPase reaction mechanism; assays for identifying tastespecific genes; quantum dots

Safety Information

WGK Germany 

3

DI-4-ANEPPS Usage And Synthesis

Description

Di-4-ANEPPS is a potentiometric fluorescent dye that can be used to measure membrane potential. It displays absorption/emission maxima of 495/705 nm, respectively. The relative fluorescence changes proportionally to membrane potential at a rate of approximately 10% per 100 mV. Di-4-ANEPPS has been used to measure membrane potential in a variety of tissue and cell types as well as artificial membranes.

Chemical Properties

Appearance: Orange-red solid
ex/em: 483/686 nm (artificial membrane)
Extinction coefficient (ε): 39,000 L??mol??1??cm??1 (methanol)

Uses

Di-4-ANEPPS is a membrane potential dye which responds to changes in membrane potential monitored by fluorescence excitation changes. It is correlated to changes in the membrane potential of a cell. Dyes and metabolites.

storage

Store at -20°C

References

[1] L M LOEW. A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations.[J]. Journal of Membrane Biology, 1992, 130 1: 1-10. DOI: 10.1007/bf00233734

Abs/Em Maxima

493/708 nm

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