4',6-Diamidino-2-phenylindole dihydrochloride Chemical Properties

Melting point:

>300°C (dec.)

storage temp. 

2-8°C

solubility 

H₂O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.

form 

powder

color 

Clear

PH

4.0-5.0 (10g/l, H2O, 20℃)

λmax

358 nm, 342 nm

BRN 

4894417

Stability:

PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months.

Biological Applications

Nuclear apoptosis assay; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer’s disease,Down’s syndrome,type II diabetes; monitoring bioaerosols

InChIKey

FPNZBYLXNYPRLR-UHFFFAOYSA-N

CAS DataBase Reference

28718-90-3(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xi,Xn

Risk Statements 

36/37/38-20/21/22

Safety Statements 

26-36-24/25-22-37/39

WGK Germany 

3

RTECS 

NL5995050

F 

3-8-10-21

HS Code 

29339900

MSDS

• Language:English Provider:SigmaAldrich

4',6-Diamidino-2-phenylindole dihydrochloride Usage And Synthesis

Description

DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.

Chemical Properties

Yellow Solid

Uses

DAPI is used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex and as a cell permeable fluorescent minor groove-binding probe for DNA. For staining DNA in agarose gels

Uses

Suitable for

• DNA staining in agarose gels
• analysis of changes in DNA during apoptosis
• detection of mycoplasma
• photofootprinting of DNA
• immunofluorescent staining of cells

DAPI has been used:-

• in rapid monitoring of microbial contamination
• in chromosomal banding technique
• in detection of apoptotic cells
• in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA
• to stain mature pollen grains(0.5 mg/ml)

Uses

4',6-Diamidino-2-phenylindole dihydrochloride is used as a cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine.It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.

General Description

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

storage

-20°C

References

[1] W. C. RUSSELL D. H W CAROL NEWMAN. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses[J]. Nature, 1975, 253 5491: 461-462. DOI:10.1038/253461a0.
[2] BETTY I. TARNOWSKI James H N F Spinale. DAPI as a useful stain for nuclear quantitation.[J]. Biotechnic & Histochemistry, 1991, 22 1: 297-302. DOI:10.3109/10520299109109990.
[3] ANA KATRINA ESTANDARTE. The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes.[J]. Scientific Reports, 2016: 31417. DOI:10.1038/srep31417.

4',6-Diamidino-2-phenylindole dihydrochloride(28718-90-3)Related Product Information

• Indole-3-butyric acid
• FORMAMIDINE HYDROCHLORIDE
• Indometacin
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• Dihydrochloride
• 1H-INDOLE-6-METHANAMINE
• Benzamidine hydrochloride
• 6-Methylindole
• 4-Methylbenzene-1-carboximidamide hydrochloride
• 4-Methylbenzylamine
• BENZAMIDINE
• 2-Phenylindole
• Acetamidine hydrochloride
• 4',6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE N-HYDRATE,4',6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE: HYDRATE,DAPI DIHYDROCHLORIDE HYDRATE,DAPI
• 1H-Indole, 2,6-dimethyl-
• 4-METHYL-BENZAMIDINE
• 2-PHENYLPYRROLE
• DAPI