BroMotriMethylaMMoniuMbiMane BroMide Chemical Properties

Melting point:

222-228°C (dec.)

storage temp. 

-20°C Freezer

solubility 

DMSO: 5 mg/mL

form 

Solid

color 

Pale Yellow to Yellow

BRN 

4630658

Stability:

Moisture, Light Sensitive - Desiccate and protect from light

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

F 

10-21

HS Code 

2933998090

MSDS

• Language:English Provider:SigmaAldrich

BroMotriMethylaMMoniuMbiMane BroMide Usage And Synthesis

Chemical Properties

BroMotriMethylaMMoniuMbiMane BroMide is Yellow Solid

Uses

BroMotriMethylaMMoniuMbiMane BroMide is a charged fluoresent sulfhydryl active reagent. Bromotrimethylammoniumbimane is a derivative of monobromobimane containing a positive charge which permits separation of its conjugates by electrophoresis or cation-exchange chromatography. The membrane permeability of this charged bimane as well as its ability to access sites within a protein may differ from that of the uncharged bimane probes. These reagents are also useful for detecting the distribution of protein thiols in cells before and after chemical reduction with disulfides. Fluorescence: max. Abs. l = 398nm, max. Em. l = 475-485 (when reacted with thiol); e x 10-3: 5.0

Biological Activity

Monobromobimanes are essentially nonfluorescent until conjugated and readily react with low molecular weight thiols. Unlike common monobromobimanes, this carries a positive charge, thus permitting separation of conjugates by electrophoresis or cation-exchange chromatography

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