STREPTAVIDIN-HRP
STREPTAVIDIN-HRP Basic information
- Product Name:
- STREPTAVIDIN-HRP
- Synonyms:
-
- STREPTAVIDIN-HRP
- SA-HRP/SA-POD
- abs956
- IHC SELECT(TM)STREPTAVIDIN-HRP CONJUGATE
- HRP-CONJUGATED STREPTAVIDIN
- HORSERADISH PEROXIDASE STREPTAVIDIN
- PEROXIDASE-STREPTAVIDIN
- STREPTAVIDIN-PEROXIDASE
- MW:
- 0
- Product Categories:
-
- Biotin/Avidin
- Glycobiology
- Glycoprotein Detection
- Phosphorylation Analysis
- Streptavidin Reagents
- Antibody Conjugates
- Detection of Biotin Labeled Glycoproteins on Western BlotsWestern Blotting
- Peroxidase ConjugatesPost-Translational Modification
- Protein Detection
- Secondary Antibodies and Labeled StreptavidinPlant Proteomics
- Streptavidin ReagentsSupplementary Products
- Mol File:
- Mol File
STREPTAVIDIN-HRP Chemical Properties
- storage temp.
- −20°C
- form
- lyophilized powder
MSDS
- Language:English Provider:SigmaAldrich
STREPTAVIDIN-HRP Usage And Synthesis
Uses
Streptavidin- Peroxidase from Streptomyces avidinii has been used for ELISA (enzyme linked immunosorbent assay) and Enzyme-Linked ImmunoSpot (ELISPOT).
General Description
Streptavidin is considered as a high-affinity biotin-binding agent, which shows resistance to extreme pH, detergents temperature, denaturants and enzymes, hence it is used in molecular biology and bionanotechnology.
Biochem/physiol Actions
Streptavidin is an antibiotic that functions by binding to and depleting the essential vitamin biotin from the surrounding environment. Because of its unique properties, streptavidin has found various applications in biological studies, including immunotherapy, immunoassays, hybridization assays, lymphocyte activation, antigen localization, and affinity chromatography.
Description
This product is useful for the detection of biotinylated proteins (1,2). Conjugation of horseradish peroxidase (HRP) to streptavidin is obtained by cross linking the amino groups on streptavidin with the carbohydrate groups on HRP.
Reactivity
All
Background
Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii for use in isolation and detection bioassays. Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins.Chemiluminescent detection systems have emerged as the best all-around detection method for use with western blots and ELISA. These detection assays eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Streptavidin-HRP is used with biotinylated proteins and specific chemiluminescent substrates to generate light signal. Streptavidin-HRP conjugates have a very high turnover rate, coupling high sensitivity with short reaction times.
References
[1] Updyke, T.V. and Nicolson, G.L. (1984) J Immunol Methods 73, 83-95.
[2] Buckie, J.W. and Cook, G.M. (1986) Anal Biochem 156, 463-72.
[3] Chaiet, L. and Wolf, F.J. (1964) Arch Biochem Biophys 106, 1-5.
[4] Reznik, G.O. et al. (1998) Proc Natl Acad Sci USA 95, 13525-30.
STREPTAVIDIN-HRPSupplier
- Tel
- 400-6206333 13167063860
- anhua.mao@aladdin-e.com
- Tel
- 400 638 7771
- sales@heowns.com
- Tel
- 0411-62910999 13889544652
- sales@meilune.com
- Tel
- 021-61415566 800-8193336
- orderCN@merckgroup.com
- Tel
- +86-17380623303
- Caroline@youngshechem.com
STREPTAVIDIN-HRP()Related Product Information
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- BCIP/NBT
- TTH DNA POLYMERASE
- dNTP Mix 25mM each
- Platinum Taq DNA Polymerase
- Hotstart Master PCR Mix (25X)
- Hotstart HiTaq PCR Mix (2X)
- dN(U)TP Mix 20/40mM each
- Hotstart HiTaq DNA Polymerase
- Taq Antibody (10U/ul)
- Abstart Master PCR Mix (25X)
- Tth One-Step RT-PCR Mix
- Hotstart HiTaq One-Step RT-PCR Mix
- AP-Streptavidin
- Abstart PCR Mix (2X)
- Hotstart HiTaq One-Step RT-PCR Mix (Heat-labile UDG)
- AVIDIN
- STREPTAVIDIN