1-Methoxy-5-methylphenazinium methyl sulfate Chemical Properties

Melting point:

172 °C

storage temp. 

Inert atmosphere,Room Temperature

solubility 

Water: 1 mg/ml

form 

powder to crystal

color 

Orange to Brown to Dark purple

InChI

InChI=1S/C14H13N2O.CH4O4S/c1-16-11-7-4-3-6-10(11)15-14-12(16)8-5-9-13(14)17-2;1-5-6(2,3)4/h3-9H,1-2H3;1H3,(H,2,3,4)/q+1;/p-1

InChIKey

MASUWVVNWALEEM-UHFFFAOYSA-M

SMILES

S([O-])(=O)(=O)OC.O(C1=CC=CC2=[N+](C3=CC=CC=C3N=C12)C)C

CAS DataBase Reference

65162-13-2(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xn

Risk Statements 

22-36/38-40

Safety Statements 

26

WGK Germany 

3

HS Code 

2933.99.8290

1-Methoxy-5-methylphenazinium methyl sulfate Usage And Synthesis

Uses

1-Methoxy-5-methylphenazinium methyl sulfate (PMS) has a redox potential of +63 mV. PMS is often used as an electron carrier for NADH tetrazolium and is a useful reagent for NAD(P)H-tetrazolium-based analytical systems. It is mainly used in the cytotoxic proliferation reagent CCK-8 experiment.

Mechanism of action

1-Methoxy-5-methylphenazinium methyl sulfate contains WST-8 [chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene)-2H-tetrazolyl monosodium salt], which is reduced to a highly water-soluble yellow formazan dye by dehydrogenase in cells under the action of the electron carrier 1-methoxy-5-methylphenazinium dimethyl sulfate. The amount of formazan generated is proportional to the number of living cells. Therefore, this property can directly analyze cell proliferation and toxicity. The main uses of this reagent are drug screening, cell proliferation assay, cytotoxicity assay, and tumour drug sensitivity test.

1-Methoxy-5-methylphenazinium methyl sulfate(65162-13-2)Related Product Information

• Vanadyl sulfate
• Methyl methacrylate
• Ammonium sulfate
• Barium sulfate
• Sodium sulfate
• Phenazine methosulfate
• Methyl 2-[2-chloro-4-fluoro-5-[(3-oxo-5,6,7,8-tetrahydro-[1,3,4]thiadiazolo[3,4-a]pyridazin-1-ylidene)amino]phenyl]sulfanylacetate
• Magnesium sulfate heptahydrate
• Aluminum sulfate
• Zinc sulfate heptahydrate
• sulfate
• 5-Hydroxyquinoxaline
• Phenazine
• 5-Methoxyquinoxaline
• 1-METHOXYPHENAZINE
• 1-HYDROXYPHENAZINE
• 1-Methoxy-5-methylphenazinium methyl sulfate
• GOLD COAST SOLUTION NO 1