L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE Chemical Properties

storage temp. 

2-8°C

solubility 

Soluble in Water (up to 5 mg/ml).

form 

solid

color 

Off-white

Stability:

Stable for 1 year from date of purchase as supplied. Solutions in distilled water may be stored at -20° for up to 1 month.

EPA Substance Registry System

L-Glutamine, N-(4-nitrophenyl)-, monohydrochloride (67953-08-6)

Safety Information

Safety Statements 

22-24/25

WGK Germany 

3

MSDS

• Language:English Provider:SigmaAldrich

L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE Usage And Synthesis

Description

L-γ-Glutamyl-p-nitroanilide (67953-08-6; GPNA) is a substrate for γ-glutamyl transpeptidase. GPNA is used for the determination of γ-glutamyl transpeptidase hydrolytic activity via spectrophotometric monitoring of p-nitroaniline released by the action of the enzyme on GPNA.1 GPNA has also been shown to be an inhibitor of glutamine uptake – solute-linked carrier family A1 member 5 (SLC1A5).2?Cellular uptake of L-glutamine (regulated by SLC1A5) is a rate-limiting step that activates mTOR and is, thus, an interesting cancer chemotherapy target.3

Uses

L-Glutamic acid gamma-(p-nitroanilide) is used in methods of treating cancers having a deregulated NRF2/KEAP1 pathway.

References

1) Meister?et al. (1981),?γ-Glutamyl transpeptidase; Methods Enzymol.,?77?237 2) Esslinger?et al. (2005),?N-gamma-aryl glutamine analogues as probes of the ASCT2 neutral amino acid transporter binding site; Bioorg. Med. Chem.,?13?1111 3) Nicklin?et al. (2012),?Bidirectional Transport of Amino Acid Regulates mTOR and Autophagy; Cell,?136?521

L-GLUTAMIC ACID GAMMA-(P-NITROANILIDE) HYDROCHLORIDE(67953-08-6)Related Product Information

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