CTC Chemical Properties

Melting point:

225 ℃

storage temp. 

2-8°C

solubility 

Soluble in water

form 

powder

color 

Off-white

λmax

450nm (of formazan)

BRN 

8954451

Biological Applications

NADPH-cytochrome P 450 reductase activity assays; detecting gamma-hydroxybutyric acid (GHB),metabolic activity,microorganisms; localizing glucose-6-phosphate dehydrogenase activity; monitoring respiratory activity; treating cancer

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26

WGK Germany 

3

F 

8

HS Code 

2933998090

MSDS

• Language:English Provider:SigmaAldrich

CTC Usage And Synthesis

Description

5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.

Uses

Redox dye producing a fluorescent formazan (only in solid state) upon reduction. It is used as cellular indicator of respiratory activity in cells, especiall for tracking P450 activity.

Uses

5-Cyano-2,3-di-p-tolyltetrazolium Chloride is a monotetrazolium redox dye that is used as a vital stain for actively respiring bacteria. Redox dye producing a fluorescent formazan (only in solid state) upon reduction; used as cellular indicator of respiratory activity in cells.

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