H-HIS-CYS-LEU-GLY-LYS-TRP-LEU-GLY-HIS-PRO-ASP-LYS-PHE-OH
H-HIS-CYS-LEU-GLY-LYS-TRP-LEU-GLY-HIS-PRO-ASP-LYS-PHE-OH Basic information
- Product Name:
- H-HIS-CYS-LEU-GLY-LYS-TRP-LEU-GLY-HIS-PRO-ASP-LYS-PHE-OH
- Synonyms:
-
- MYELIN PROTEOLIPID PROTEIN (139-151) (DEPALMITOYLATED) (HUMAN, BOVINE, DOG, MOUSE, RAT)
- MYELIN PROTEOLIPID PROTEIN (139-151) (HUMAN, BOVINE, DOG, MOUSE, RAT)
- LIPOPHILIN (139-151) (DEPALMITOYLATED) (HUMAN, BOVINE, DOG, MOUSE, RAT)
- HIS-CYS-LEU-GLY-LYS-TRP-LEU-GLY-HIS-PRO-ASP-LYS-PHE
- H-HIS-CYS-LEU-GLY-LYS-TRP-LEU-GLY-HIS-PRO-ASP-LYS-PHE-OH
- H-HIS-CYS-LEU-GLY-LYS-TRP-LEU-GLY-HIS-PRO-ASP-LYS-PHE-OH (HUMAN, BOVINE, DOG, MOUSE, RAT)
- PLP (139-151) (DEPALMITOYLATED) (HUMAN, BOVINE, DOG, MOUSE, RAT)
- PLP (HUMAN, BOVINE, DOG, MOUSE, RAT)
- CAS:
- 131334-43-5
- MF:
- C72H104N20O16S
- MW:
- 1537.79
- Mol File:
- 131334-43-5.mol
H-HIS-CYS-LEU-GLY-LYS-TRP-LEU-GLY-HIS-PRO-ASP-LYS-PHE-OH Chemical Properties
- Boiling point:
- 1897.4±65.0 °C(Predicted)
- Density
- 1.332±0.06 g/cm3(Predicted)
- pka
- 3.49±0.10(Predicted)
- form
- White to off-white powder.
- color
- White to off-white
- Sequence
- H-His-Cys-Leu-Gly-Lys-Trp-Leu-Gly-His-Pro-Asp-Lys-Phe-OH
H-HIS-CYS-LEU-GLY-LYS-TRP-LEU-GLY-HIS-PRO-ASP-LYS-PHE-OH Usage And Synthesis
Uses
PLP (139-151) is amino acid residue 139 to 151 of myelin proteolipid protein (PLP) used to induce experimental autoimmune encephalomyelitis (EAE).
in vivo
PLP (139-151) can be used in animal modeling to create relapsing-remitting experimental autoimmune encephalomyelitis (RR-EAE) models. The pro-inflammatory cytokine IL-17 plays a crucial role in the PLP (139-151)-induced encephalomyelitis process. After subcutaneous injection of PLP (139-151) into mice, CD4 cells that specifically respond to PLP (139-151) and release pro-inflammatory cytokines migrate to and accumulate in the damaged central nervous system (CNS) regions[4][5].
Administration: 100 μg ? sc ? single dose
2. One day after the subcutaneous injection of PLP (139-151), perform an intraperitoneal injection of 100 μl (2 ng/μl, dissolved in phosphate-buffered saline (PBS)) of Bordetella pertussis toxin (BPX) to further enhance the mouse's immune response to PLP (139-151).
3. Monitor the mice daily for changes in body weight and clinical status. Perform neurological evaluations using a clinical score (CS) system with the following scale: 0 for no abnormalities; 0.5 for a drooping tail tip; 1 for a limp tail; 1.5 for hind limb dysfunction; 2 for hind limb weakness; 2.5 for dragging hind limbs; 3 for hind limb paralysis; 3.5 for complete hind limb paralysis with the mouse unable to right itself when placed on its side; and 4 for complete hind limb paralysis and partial front limb paralysis.
4. Isolate mice with early clinical symptoms of encephalomyelitis in separate cages with easier access to food and water. Dehydrated mice should receive an intraperitoneal injection of saline solution at least once a day.
Histological Changes: Resulted iron deposits in the cerebellum, medulla, and spinal cord of the mice.
Molecular Changes: Enhanced the immune signals of TSPO and Iba1 (markers of microglia and macrophage activation) in immunohistochemical results, indicating that microglia and macrophages were activated.
References
[1] Kuchroo VK, et al. Induction of experimental allergic encephalomyelitis by myelin proteolipid-protein-specific T cell clones and synthetic peptides. Pathobiology. 1991;59(5):305-12. DOI:10.1159/000163668
[2] Sobel RA, et al. Acute experimental allergic encephalomyelitis in SJL/J mice induced by a synthetic peptide ofmyelin proteolipid protein. J Neuropathol Exp Neurol. 1990 Sep;49(5):468-79. DOI:10.1097/00005072-199009000-00002
[3] Bebo BF Jr, et al. Male SJL mice do not relapse after induction of EAE with PLP 139-151. J Neurosci Res. 1996 Sep 15;45(6):680-9. DOI:10.1002/(SICI)1097-4547(19960915)45:63.0.CO;2-4
[4] Coda AR, et al. In vivo imaging of CNS microglial activation/macrophage infiltration with combined [18F]DPA-714-PET and SPIO-MRI in a mouse model of relapsing remitting experimental autoimmune encephalomyelitis. Eur J Nucl Med Mol Imaging. 2021 Jan;48(1):40-52. DOI:10.1007/s00259-020-04842-7
[5] Turvey ME, et al. Quantitative proteome profiling of CNS-infiltrating autoreactive CD4+ cells reveals selective changes during experimental autoimmune encephalomyelitis. J Proteome Res. 2014 Aug 1;13(8):3655-70. DOI:10.1021/pr500158r
[6] Zhang H, et al. Intrinsic and induced regulation of the age-associated onset of spontaneous experimental autoimmune encephalomyelitis. J Immunol. 2008 Oct 1;181(7):4638-47. DOI:10.4049/jimmunol.181.7.4638
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