4-THIOURACIL Chemical Properties

Melting point:

295 °C (dec.) (lit.)

Density 

1.368 (estimate)

refractive index 

1.7000 (estimate)

storage temp. 

Store at -20°C

solubility 

1 M NaOH: soluble50mg/mL

form 

Powder

pka

5.38±0.20(Predicted)

color 

Yellow

CAS DataBase Reference

591-28-6(CAS DataBase Reference)

Safety Information

Hazard Codes 

Xn

Risk Statements 

20/21/22

Safety Statements 

36

WGK Germany 

3

HS Code 

29335990

MSDS

• Language:English Provider:SigmaAldrich
• Language:English Provider:ACROS

4-THIOURACIL Usage And Synthesis

Description

4-Thiouracil is a site-specific, photoactivatable probe used to detect RNA structures and nucleic acid-nucleic acid contacts. It absorbs ultraviolet light >300 nm and, in the presence of oxygen, acts as an energy donor to produce singlet oxygen by triplet-triplet energy transfer. The highly reactive oxygen species then reacts readily with 4-thiouracil, leading to the production of uracil and uracil-6-sulfonate, which is fluorescent at a wavelength of ~390 nm. 4-Thiouracil is used as a T. gondii uracil phosphoribosyltransferase substrate to produce 4-thiouridine monophosphate, which can ultimately be incorporated into RNA.

Chemical Properties

yellow powder

Uses

4-Thiouracil is suitable reagent employed in Schneider′s media for the embryos during RNA extraction. It may be employed as reagent for the Northwestern blotting technique.

Uses

4-Thiouracil is a derivative of Uracil (U801000), which is a nitrogenous base in RNA nucleic acid. 4-Thiouracil is used for tagging in cell type-specific RNA isolation from intact complex tissues.

Synthesis Reference(s)

Synthesis, p. 256, 1987 DOI: 10.1055/s-1987-27906

4-THIOURACIL(591-28-6)Related Product Information

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