FPG PROTEIN Chemical Properties

storage temp. 

-20°C

form 

buffered aqueous glycerol solution

biological source

Escherichia coli

Specific Activity

>20,000units/mg protein

Safety Information

Hazard Codes 

Xi

Risk Statements 

36/37/38

Safety Statements 

26-36

WGK Germany 

1

FPG PROTEIN Usage And Synthesis

Uses

Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

General Description

Fpg contains two domains separated by a flexible hinge.

Biochem/physiol Actions

Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.