PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Chemical Properties

Density 

1.29 g/mL at 20 °C

Flash point:

79 °C

storage temp. 

2-8°C

form 

Liquid

PH

7.7-8.3(H2O-phase, after extraction with H2O, 1:1)

Water Solubility 

Soluble in water.

λmax

λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1

Absorption

cut-off at 360nm

Safety Information

Hazard Codes 

T

Risk Statements 

23/24/25-34-40-48/20/21/22-68-63

Safety Statements 

26-36/37/39-45

RIDADR 

UN 2810 6.1/PG 2

WGK Germany 

3

F 

8-10-23

HazardClass 

8

HS Code 

29420000

PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Usage And Synthesis

Chemical Properties

clear yellow solution

Uses

Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA.

Biological Activity

When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.

PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1(136112-00-0)Related Product Information

• Barium hydroxide
• Lysozyme
• Ethanol
• Acetic acid
• Chloroform