Z-ARG-LEU-ARG-GLY-GLY-AMC Chemical Properties

Density 

1.40±0.1 g/cm3(Predicted)

storage temp. 

-20°C

solubility 

DMSO: Sparingly Soluble: 1-10 mg/ml

pka

11.02±0.46(Predicted)

form 

film or powder

color 

white to beige

Sequence

{Z}-Arg-Leu-Arg-Gly-Gly-{AMC}

Z-ARG-LEU-ARG-GLY-GLY-AMC Usage And Synthesis

Uses

Z-Arg-Leu-Arg-Gly-Gly-AMC is a peptide substrate of SARS-CoV PLpro[1].

Biological Activity

Z-RLRGG-AMC is a fluorogenic substrate for the MERS-CoV, SARS-CoV, SARS-CoV-2 papain-like protease (PLpros), deubiquitinating enzyme isopeptidase T (IPaseT) and other ubiquitin C-terminal hydrolases, which consists of the Ub and ISG15 C-terminal sequences. Z-RLRGG-AMC excitation wavelength is 340 nm, and emission wavelength is 460 nm.

References

[1] Schalk F, et, al. GNPS-guided discovery of xylacremolide C and D, evaluation of their putative biosynthetic origin and bioactivity studies of xylacremolide A and B. RSC Adv. 2021 May 24;11(31):18748-18756. DOI:10.1039/d1ra00997d
[2] ROSS L. STEIN  Francesco M  Zhijian Chen. Kinetic studies of isopeptidase T: modulation of peptidase activity by ubiquitin[J]. Biochemistry Biochemistry, 1995, 34 39: 12616-12623. DOI: 10.1021/bi00039a017

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