SBA, Soybean agglutinin Chemical Properties

storage temp. 

−20°C

SBA, Soybean agglutinin Usage And Synthesis

Uses

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

SBA, Soybean agglutinin()Related Product Information

• Soybean Flour